The mechanism identification of environmental selective bone induction/suppression of BMP-2

• Project/Area Number: 25463050
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Okayama University
• Principal Investigator: Nawachi Kumiko 岡山大学, 大学病院, 助教 (10379787)
• Co-Investigator(Kenkyū-buntansha): KUBOKI TAKUO 岡山大学, 大学院医歯薬学総合研究科, 教授 (00225195) ; SONOYAMA WATARU 岡山大学, 岡山大学病院, 講師 (40325121) ; ONO MITSUAKI 岡山大学, 大学院医歯薬学総合研究科, 助教 (60613156) ; AKIYAMA KENTARO 岡山大学, 岡山大学病院, 講師 (70423291) ; SHINKAWA SHIGEHIKO 岡山大学, 岡山大学病院, 医員 (10727465)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 骨形成 / BMP-2 / 骨髄 / 口腔インプラント / オッセオインテグレーション / rhBMP-2 / 骨吸収

Outline of Final Research Achievements

Recombinant human BMP-2 (rhBMP-2) has been widely used to treat bone defects or bone-associated diseases in human. However, recently our research group found that rhBMP-2 inhibited bone formation in in the bone marrow space. The purpose of this research was to elucidate the mechanism of environmental selective bone induction/suppression of BMP-2. In the mice model, BMP-2 could not induce the bone formation in the bone marrow rich environment. Moreover, large animal model experiments revealed that rhBMP-2 were suitable for operative procedure in the bone marrow poor environment, such as socket lift and socket preservation.

Research Products

• [Journal Article] Efficient bone formation in a swine socket lift model using Escherichia coli-derived recombinant human bone morphogenetic protein-2 adsorbed in β-tricalcium phosphate. (2014)
  • Author(s): Mitsuaki Ono, Wataru Sonoyama, Katushi Yamamoto, Yasutaka Oida, Kentaro Akiyama, Shigehiko Shinkawa, Ryu Nakajima, Hai Than Pham, Emilio Satoshi Hara, Takuo Kuboki
  • Journal Title: Cells, Tissues, Organs Volume: 199 Issue: 4 Pages: 249-255
  • DOI: 10.1159/000369061
  • Peer Reviewed / Open Access
• [Journal Article] The BMP2 antagonist inhibitor L51P enhances the osteogenic potential of BMP2 by simultaneous and delayed synergism. (2014)
  • Author(s): HM. Khattab, M. Ono, W. Sonoyama, Y. Oida, S. Shinkawa, Y. Yoshioka, K. Maekawa, Y. Tabata, K. Sugama, W. Sebald, T. Kuboki.
  • Journal Title: Bone Volume: 18 Pages: 165-173
  • DOI: 10.1016/j.bone.2014.09.011
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Regeneration of calvarial defects with Escherichia coli-derived rhBMP-2 adsorbed in PLGA membrane (2013)
  • Author(s): Ono M, Sonoyama W, Nema K, Hara ES, Oida Y, Pham HT, Yamamoto K, Hirota K, Sugama K, Sebald W, Kuboki T
  • Journal Title: Cells Tissues Organs Volume: 198(5) Issue: 5 Pages: 367-76
  • DOI: 10.1159/000356947
  • Peer Reviewed
• [Presentation] BMP2変異体L51Pは BMPネガティブ・フィードバック機構を制御することでBMP2誘導性骨芽細胞分化および骨形成を促進する (2015)
  • Author(s): 大野充昭，Khattab H，笈田育尚，Sebald W，窪木拓男
  • Organizer: 第45回公益社会法人日本口腔インプラント学会学術大会
  • Place of Presentation: 岡山，日本
  • Year and Date: 2015-09-22
• [Presentation] 骨髄腔内においてBMP-2は骨形成を抑制する (2015)
  • Author(s): 植田淳二，笈田育尚，大野充昭，Emilio Hara，Ha Naguyen，窪木拓男
  • Organizer: 第1回日本骨免疫学会
  • Place of Presentation: 沖縄，日本
  • Year and Date: 2015-07-01
• [Presentation] Efficient bone formation in a socket-lift model in swine using E.coli-derived rhBMP-2 in combination with β-TCP. (2013)
  • Author(s): Nakajima R, Sonoyama W, Ono M, Oida Y, Shinkawa S, Akiyama K, Maekawa K, Yamamoto K, Hirota K, Sugama K, Kuboki T.
  • Organizer: Efficient bone formation in a socket-lift model in swine using E.coli-derived rhBMP-2 in combination with β-TCP.
  • Place of Presentation: Jeju (Korea)