| Project/Area Number |
25463053
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
Chosa Naoyuki 岩手医科大学, 歯学部, 講師 (80326694)
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| Co-Investigator(Renkei-kenkyūsha) |
Ishisaki Akira 岩手医科大学, 歯学部, 教授 (20356439)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 歯周組織再生 / 間葉系幹細胞 / 炎症性サイトカイン / アンタゴニスト / 細胞治療 / ケモカイン |
|---|
| Outline of Final Research Achievements |
Mesenchymal stem cells (MSCs) are migrated into the inflammatory tissues by chemokines, such as SDF-1 and MCP-1, following which the effects as anti-inflammatory and tissue repair. In this study, we investigate the interaction with MSCs and periodontal ligament fibroblasts (PDL-Fs), and then examine the role of the MSCs in periodontitis accompanied by tissue disruption. Inflammatory cytokines such as IL-1β, IL-6, and TNF-α were promoted the expression of a chemokine, MCP-1, in the PDL-Fs. Expression of MSC marker such as Sca-1 and CD44 were enhanced in MSCs that are directly co-cultured with PDL-Fs. Importantly, in conjunction with suppression of a pro-inflammatory cytokine, IL-6, expression and promotion of anti-inflammatory cytokines, IL-10 and TGF-β, expression were observed. These results were suggested that anti-inflammatory effects of the MSCs is enhances, which interacts with the periodontal tissue cells by cell-cell adhesion in periodontitis.
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