The effect of angiogenesis induction on vertical bone augmentation

• Project/Area Number: 25463056
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Nihon University
• Principal Investigator: SATO Shuichi 日本大学, 歯学部, 教授 (50225942)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 血管新生 / 骨再生 / 垂直方向 / ラット / 骨増生 / 増殖因子 / 幹細胞

Outline of Final Research Achievements

The purpose of these studies were investigated the effect of angiogenesis induction on vertical bone augmentation. First study showed basic fibroblast growth factor(FGF-2) promoted angiogenesis earlier on rat non-critical and critical bone defects.The second study evaluated angiogenesis and bone augmentation with rat calvarila guided bone agumentation (GBA) model using micro-CT.The results showed angiogenesis began at sites from brain arteries and gradually increased at bone marrow penetration to vertical direction. The bone augmentation enhanced angiogenesis in rat calvaria GBA model. The third study investigated effect of platelet-derived growth factor (PDGF) on rat calvarial GBA model. The results showed 0.03% PDGF in collagen sponge and chitosan sponge enhanced vertical bone formation on rat calvarium.

Research Products

• [Journal Article] ラットGBAモデルにおける骨髄穿通孔の大きさが骨増生および血管新生に及ぼす影響 (2016)
  • Author(s): 佐藤暢亮
  • Journal Title: 日大歯学 Volume: 90
• [Journal Article] Melatonin enhances vertical bone augmentation in rat calvaria secluded spaces (2015)
  • Author(s): Shino H, Hasuike A, Arai Y, Honda M, Isokawa K, Sato S
  • Journal Title: Med Oral Patol Oral Cir Bucal Volume: 21 Pages: 122-126
  • DOI: 10.4317/medoral.20904
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 再生医療を応用した歯周治療の現状 (2015)
  • Author(s): 佐藤秀一
  • Journal Title: 日大歯学 Volume: 89 Pages: 93-99
• [Journal Article] Effects of intermittent administration on parathyroid horomone on bone augmentation in rat calvarium (2015)
  • Author(s): Tsunori K, Sato S, Hasuike A, Manaka S, Shino H, Sato N, Kubota T, Arai Y, Ito K, Miyazaki M.
  • Journal Title: Implant Dent Volume: 24 Pages: 142-148
  • DOI: 10.1097/id.0000000000000228
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Basic fibroblast growth factor angiogenesis in bone regeneration within non-critical-sized bone defects in rat calvaria (2014)
  • Author(s): Kigami R, Sato S, Tsuchiya N, Sato N, Suzuki D, Arai Y, Ito K, Ogiso B
  • Journal Title: J Oral Sci Volume: 56 Pages: 17-22
  • Peer Reviewed
• [Journal Article] FGF-2 angiogenesis in bone regeneration within critical-sized bone defects in rat calvaria, (2013)
  • Author(s): Kigami R, Sato S, Tsuchiya N, Yoshimaki T, Arai Y, Ito K
  • Journal Title: Implant Dent Volume: 22 Issue: 4 Pages: 422-427
  • DOI: 10.1097/id.0b013e31829d19f0
  • Peer Reviewed / Open Access
• [Journal Article] 歯周組織再生および骨再生における骨移植材の現状 (2013)
  • Author(s): 佐藤秀一，伊藤公一
  • Journal Title: 日本歯周病学会誌 Volume: 55 Pages: 300-311
• [Presentation] ラット水平性骨欠損モデルに対する脱分化脂肪細胞を用いた歯周組織再生治療の検討 (2015)
  • Author(s): 鈴木大悟，秋田大輔，井口慎也，河野英輔，森谷良智，鳥海拓，磯川桂太郎，新井嘉則，本田雅規，佐藤秀一
  • Organizer: 第58回秋季日本歯周病学会
  • Place of Presentation: アクトシティ浜松（静岡県浜松市）
  • Year and Date: 2015-09-12
• [Presentation] マウス歯周炎モデルに対する骨髄間質細胞を用いた歯周炎抑制の検討 (2015)
  • Author(s): 井口慎也，河野英輔，鈴木大悟，篠弘道，秋山浩教，鳥海拓，磯川桂太郎，新井嘉則，本田雅規，佐藤秀一
  • Organizer: 第58回秋季日本歯周病学会
  • Place of Presentation: アクトシティ浜松（静岡県浜松市）
  • Year and Date: 2015-09-12
• [Presentation] ラットGBAモデルにおける血管新生の動態 (2015)
  • Author(s): 佐藤暢亮，菅井健二，江田昌弘，西田哲也，山田豊，吉沼直人，菅野直行，佐藤秀一，新井嘉則
  • Organizer: 第58回春季日本歯周病学会
  • Place of Presentation: 幕張メッセ（千葉県千葉市）
  • Year and Date: 2015-05-15
• [Presentation] Effects of systematic parathyroid hormone(1-34) on bone augmented using a rat calvarium model (2014)
  • Author(s): Tsunori K, Sato S, Shino S, Takano M, Akutagawa H, Ito K
  • Organizer: 100th American Academy of Periodontology
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2014-09-19 – 2014-09-22
• [Presentation] Effects of various dosage of nicotine on guided bone augmentation (2014)
  • Author(s): Sato S, Saito Y, Mizumura Y, Masuda H, Nishida T, Sugano N, Arai Y, Ito k
  • Organizer: 100th American Academy of Periodontology
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2014-09-19 – 2014-09-22
• [Presentation] ラットの骨増生におけるメラトニンの効果 (2014)
  • Author(s): 篠 弘道，佐藤秀一，蓮池 聡，津徳亮成，佐藤暢亮，井口慎也，新井嘉則，伊藤公一，小木曾文内
  • Organizer: 第140回日本歯科保存学会春季学術大会
  • Place of Presentation: 滋賀県立芸術劇場びわ湖ホール（滋賀県大津市）
  • Year and Date: 2014-06-20
• [Presentation] ラクトフェリンの全身投与はラット頭頂骨内側性骨欠損の骨再生を促進する (2014)
  • Author(s): 吉巻友裕，佐藤秀一，津徳亮成，篠 弘道，新井嘉則，伊藤公一
  • Organizer: 第57回春季日本歯周病学会学術大会
  • Place of Presentation: 長良川国際会議場（岐阜県瑞穂市）
  • Year and Date: 2014-05-24
• [Presentation] PTH(1-34)の間歇投与によるラットGBAモデルにおける骨増生の影響 (2014)
  • Author(s): 津徳亮成，佐藤秀一，佐藤暢亮，吉巻友裕，蓮池 聡，山田 豊，新井嘉則，伊藤公一
  • Organizer: 第57回春季日本歯周病学会学術大会
  • Place of Presentation: 長良川国際会議場（岐阜県瑞穂市）
  • Year and Date: 2014-05-24
• [Presentation] 骨外側方向の骨増生における血管新生のエックス線・組織学的観察 (2014)
  • Author(s): 佐藤暢亮，佐藤秀一，長嶋麻美，新井嘉則，小木曾文内
  • Organizer: 第66回日本大学歯学会学術大会
  • Place of Presentation: 日本大学歯学部大講堂（東京都千代田区）
  • Year and Date: 2014-05-18
• [Presentation] 骨外側方向における骨再生に関する研究と今後の展望 (2013)
  • Author(s): 佐藤秀一，佐藤伸亮，篠 弘道，井口慎也，鈴木大悟，河野英輔，本田雅規
  • Organizer: 日本大学先端バイオフォーラム
  • Place of Presentation: 日大会館，東京
• [Presentation] ラットGBAモデルにおける血管新生のマイクロCTによる観察 (2013)
  • Author(s): 佐藤伸亮，佐藤秀一，宇田川麻美，篠 弘道，新井嘉則，伊藤公一
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 秋田県総合生活文化会館(秋田）
• [Presentation] Effects of FGF-2 guided bone augmentation beyond the skeletal envelope within a plastic cap in the rat calvarium (2013)
  • Author(s): Kigami R, Sato S, Yoshimaki T, Arai Y, Ito K
  • Organizer: EAO
  • Place of Presentation: Dublin, Ireland