Studies on the molecular mechanism of hyposalivation after irradiation, focusing on aquaporin-5, for clinical applications

• Project/Area Number: 25463068
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Gunma University
• Principal Investigator: SUSA Taketo 群馬大学, 医学(系)研究科(研究院), 研究員 (10622558)
• Co-Investigator(Kenkyū-buntansha): YOKOO Satoshi 群馬大学, 医学系研究科, 教授 (00322206) ; MATSUZAKI Toshiyuki 群馬大学, 医学系研究科, 教授 (30334113)
• Research Collaborator: TANIGUCHI Akie
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 放射線照射 / 唾液腺 / アクアポリン / ピロカルピン / アクアポリン5 / イソプロテレノール

Outline of Final Research Achievements

We analyzed the relationships between the hyposalivation after irradiation to head and neck region and the expression and subcellular distribution of water channel aquaporin-5 that plays an important role in water secretion in the salivary gland acinar cells using rats and mice. Single dose irradiation of 15 gray to these animals caused apparent reduction of saliva in 1 month and the reduction continued until 2 months. No apparent change in either the expression or subcellular distribution of aquaporin-5 was found in the parotid glands, although there were some abnormal cells having larger nuclei. Pretreatment with pilocarpine did not show apparent protective effect against hyposalivation in our current study.

Research Products

• [Journal Article] The distribution and function of aquaporins in the kidney: resolved and unresolved questions. (2016)
  • Author(s): Toshiyuki Matsuzaki, Tomoyuki Yaguchi, Kinue Shimizu, Aoi Kita, Kenichi Ishibashi, Kuniaki Takata
  • Journal Title: Anatomical Science International Volume: 印刷中 Issue: 2 Pages: 1-13
  • DOI: 10.1007/s12565-016-0325-2
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Regulation of Aquaporins by vasopressin in the kidney (2015)
  • Author(s): Ikeda M, Matsuzaki T
  • Journal Title: Vitamins and Hormones Volume: 98 Pages: 307-337
  • DOI: 10.1016/bs.vh.2014.12.008
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effects of Repeated Administration of Pilocarpine and Isoproterenol on Aquaporin-5 Expression in Rat Salivary Glands (2013)
  • Author(s): Susa T, Sawai N, Aoki T, Iizuka-Kogo A, Kogo H, Negishi A, Yokoo S, Takata K, Matsuzaki T
  • Journal Title: ACTA HISTOCHEMICA ET CYTOCHEMICA Volume: 46 Issue: 6 Pages: 187-197
  • DOI: 10.1267/ahc.13037
  • NAID: 130003381873
  • ISSN: 0044-5991, 1347-5800
  • Peer Reviewed
• [Presentation] 唾液腺腺房細胞で唾液分泌に必要とされるAQP5、NKCC1、およびTMEM16Aの絶食による発現量変化の検討 (2016)
  • Author(s): 谷口明慧，黒川潤，本間実，向後寛，向後晶子，横尾聡，松崎利行
  • Organizer: 第121回日本解剖学会総会・全国学術集会
  • Place of Presentation: ビッグパレットふくしま（福島県郡山市）
  • Year and Date: 2016-03-29
• [Presentation] 口腔がん治療時の口腔不快症状と漢方－患者のQOL維持・向上を目指した漢方薬の適応－ (2015)
  • Author(s): 五味暁憲，清水崇寛，横尾聡
  • Organizer: 第66回日本東洋医学会学術総会
  • Place of Presentation: 富山国際会議場（富山県富山市）
  • Year and Date: 2015-06-14
• [Presentation] Tissue distribution of water channel proteins (aquaporins and relatives) (2015)
  • Author(s): Toshiyuki Matsuzaki, Kuniaki Takata
  • Organizer: The second world congress on water channel proteins (aquaporins and relatives)
  • Place of Presentation: “Babes-Bolyai” University, Cluj-Napoca, Romania
  • Year and Date: 2015-05-07
  • Int'l Joint Research / Invited
• [Presentation] ラット、マウスの腎臓近位尿細管におけるアクアポリンの分布局在の再検討 (2014)
  • Author(s): 松崎利行，矢口知征，清水絹恵
  • Organizer: 第55回日本組織細胞化学会総会・学術集会
  • Place of Presentation: 松本市中央公民館（長野県・松本市）
  • Year and Date: 2014-09-28
• [Presentation] 腎臓における水チャネル (2014)
  • Author(s): 松崎利行
  • Organizer: 第61回北関東医学会総会
  • Place of Presentation: 群馬大学（群馬県・前橋市）
  • Year and Date: 2014-09-26
  • Invited
• [Presentation] アクアポリンの発現と機能的意義―下垂体の幹・前駆細胞ニッチにおける発現も含め－ (2014)
  • Author(s): 松崎利行，澤井信彦，小澤一史
  • Organizer: 日本下垂体研究会第29回学術集会
  • Place of Presentation: 八王子セミナーハウス（東京都・八王子市）
  • Year and Date: 2014-08-09
  • Invited
• [Presentation] ラット唾液線における細胞膜水チャネル-アクアポリン5の発現調節に関する検討
  • Author(s): 須佐岳人，澤井信彦，向後晶子，青木武生，向後 寛，横尾 聡，高田邦昭，松崎利行
  • Organizer: 第54回日本組織細胞化学会総会・学術集会
  • Place of Presentation: 航空会館（東京都・港区）
• [Presentation] 細胞膜水チャネル‐アクアポリン5のラット唾液腺における発現調節に関する検討
  • Author(s): 須佐岳人，根岸明秀，横尾聡
  • Organizer: 第58回日本口腔外科学会総会・学術大会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡（福岡県・福岡市）
• [Presentation] ピロカルピンまたはイソプロテレノール投与による唾液腺アクアポリン5発現量の変化
  • Author(s): 須佐岳人，澤井信彦，向後晶子，青木武生，向後 寛，横尾 聡，高田邦昭，松崎利行
  • Organizer: 第119回日本解剖学会総会・全国学術集会
  • Place of Presentation: 自治医科大学キャンパス（栃木県・下野市）
• [Book] 組織細胞化学2014 (2014)
  • Author(s): 松崎利行
  • Total Pages: 230
  • Publisher: 日本組織細胞化学会