| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
The purpose of this study was to compare the effect of some cryopreservation methods on periodontal regeneration of rat teeth in vitro. The maxillary molars of 4-week old Wister rats were extracted and frozen by gradual freezing method using a programmed freezer or super cooling-rapid freezing method for 1, 3, 6, 9, 12 months, and then thawed in a water bath at 37°C. The periodontal ligament cells were isolated and cultured. Cell proliferation of super cooling-rapid freezing group was slightly higher than gradual freezing one, but there was no significant difference.
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