Proliferating activity of cryopreserved periodontal ligament cells

• Project/Area Number: 25463073
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Niigata University
• Principal Investigator: ONO Yukiko 新潟大学, 医歯(薬)学総合研究科, 非常勤講師 (80345511)
• Co-Investigator(Kenkyū-buntansha): YOSHIZAWA Michiko 新潟大学, 医歯学総合病院, 講師 (60303137)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 凍結保存 / 歯 / 歯根膜 / 幹細胞 / 培養 / 細胞工学 / 再生医療 / 低酸素

Outline of Final Research Achievements

The purpose of this study was to compare the effect of some cryopreservation methods on periodontal regeneration of rat teeth in vitro. The maxillary molars of 4-week old Wister rats were extracted and frozen by gradual freezing method using a programmed freezer or super cooling-rapid freezing method for 1, 3, 6, 9, 12 months, and then thawed in a water bath at 37°C. The periodontal ligament cells were isolated and cultured. Cell proliferation of super cooling-rapid freezing group was slightly higher than gradual freezing one, but there was no significant difference.

Research Products

• [Journal Article] Autotransplantation or replantation of cryopreserved teeth: a case series and literature review (2013)
  • Author(s): Yoshizawa M, Koyama T, Izumi N, Niimi K, Ono Y, Ajima H, Funayama A, Mikami T, Kobayashi T, Ono K, Takagi R, Saito C
  • Journal Title: Dent Traumatol Volume: Epub ahead of print Issue: 1 Pages: 71-75
  • DOI: 10.1111/edt.12039
  • Peer Reviewed