Application to oral cancer treatment of nanobubbles and sonication
Project/Area Number |
25463096
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Surgical dentistry
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Research Institution | Kyushu Dental College |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | ソノポレーション / ナノバブル / マイクロバブル / ドラッグデリバリー / 口腔癌 / 癌 / ドラッグデリバリーシステム / バブルリポソーム / 遺伝子導入 |
Outline of Final Research Achievements |
Ultrasound creates transient permeability of cell membranes in the presence of nanobubbles or microbubbles, and foreign molecules can easily enter cells, which is known as ‘sonoporation’. We developed anti-Epitherial Growth Factor Receptor (EGFR) antibody conjugated MBs (EGFR-MB). We examined the effect of EGFR-MB on oral squamous cell carcinoma. MBs were conjugated with an anti-EGFR antibody, and were entrapped ultrasound contrast gas. Ca9-22, a human gingival carcinoma cell line, were exposed to ultrasound with EGFR-MB and anti-cancer drug bleomycin (BLM). Sonoporation and EGFR-MB had a strong cytotoxic effect on Ca9-22 cells at the low dose of BLM. In addition, sonoporation and EGFR-MB remarkably increased the population of apoptotic cells even at a low dose BLM.Treatment with EGFR-MB and ultrasound make it possible to administer drugs into the cells more efficiently and specifically, suggesting a novel application for chemotherapy treatment for oral squamous cell carcinoma.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] Maxillary single-jaw surgery combining Le Fort 1 and modified horseshoe osteotomies for the correction of maxillary excess.2016
Author(s)
Tominaga, K., Habu, M., Iwanaga, K., Kodama, M., Tususima, H., Kokuryo, S., Miyamoto, I., Fukudome, Y., Yosioka, I.
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Journal Title
Int J Oral Maxillofac Surg
Volume: 45
Issue: 2
Pages: 194-199
DOI
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Peer Reviewed
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[Presentation] Anti-inflammatory effect of IL-1 receptor antagonist gene transfection into synovial cells using sonoporation.
Author(s)
Nagao,F.,Tsuzisawa,K.,Iwanaga,K.,Yoshioka,I.,Nishihara,T. and Tominaga,K
Organizer
7th International Symposium on Nanomedicine(ISNM)
Place of Presentation
Kitakyusyu
Related Report
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