| Project/Area Number |
25463118
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Surgical dentistry
|
|---|
| Research Institution | Meikai University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
KUSAMA Kaoru 明海大学, 歯学部・病態診断治療学講座病理学分野, 教授 (20130479)
SAKASHITA Hideaki 明海大学, 歯学部・病態診断治療学講座口腔顎顔面外科学2分野, 教授 (10178551)
MIYAZAKI Yuji 明海大学, 歯学部・病態診断治療学講座病理学分野, 助教 (40526547)
YAMADA Tsutomu 日本大学, 医学部・病態病理学系人体病理学分野, 講師 (40182539)
IDE Fumio 明海大学, 歯学部・病態診断治療学講座病理学分野, 臨床教授 (50147617)
|
|---|
| Research Collaborator |
INOUE Harumi
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Project Status |
Completed (Fiscal Year 2015)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | Epstein-Barr virus (EBV) / EBNA-2 / LMP-1 / EBER / OSCC / Epithelial dysplasia / Periodontitis / ヒト口腔扁平上皮癌 / 歯周病 |
|---|
| Outline of Final Research Achievements |
In the present study we detected the EBV genome and latent EBV gene expression in normal mucosal epithelia, epithelial dysplasia and oral squamous cell carcinoma (OSCC) to clarify whether EBV is involved in carcinogenesis of the oral cavity. We examined 333 formalin-fixed, paraffin-embedded tissue samples (morphologically normal oral mucosa 30 samples, gingivitis 32, tonsillitis 17, oral epithelial dysplasia 83, OSCC 150 and NPC 21). EBV latent infection genes (EBNA-2, LMP-1) were detected not only in OSCC but also in severe epithelial dysplasia, mild to moderate epithelial dysplasia, gingivitis and normal mucosa. Furthermore, the intensity of EBV latent infection gene expression (EBER, LMP-1) was significantly higher in severe epithelial dysplasia than in OSCC. These results suggest that EBV latent infection genes and their increased expression in severe epithelial dysplasia might play an important role in the dysplasia-carcinoma sequence in the oral cavity.
|
