| Project/Area Number |
25463123
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Hyogo Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KISHIMOTO Hiromitsu 兵庫医科大学, 医学部, 教授 (30291818)
NAKANO Yoshiro 兵庫医科大学, 医学部, 教授 (30360267)
URADE Masahiro 兵庫医科大学, 医学部, 特別招聘教授 (70104883)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 口腔癌 / がん化学療法 / シスプラチン / 獲得耐性 / ヒッポシグナル / YAP / 口腔扁平上皮癌 / ヘッジホッグシグナル / 抗がん剤耐性 / Hippo pathway |
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| Outline of Final Research Achievements |
Although CDDP are used widely to treat oral squamous cell carcinoma (OSCC), many patients display acquired resistance. We investigated the correlation between YAP expression and the sensitivity to CDDP. At first, we established three CDDP resistant cell lines, and examined the expression levels of ATP7B, which are typically considered as the mechanism of CDDP resistance, and Hippo pathway related protein. We examined immunocytochemically the cellular localization of YAP. Expression levels of YAP were no significant difference between parental and resistant cell, but expression level of phospholyrated YAP of resistant cells was clearly decreased. Furthermore, translocation from cytoplasm to nucleus of YAP were observed compared with parental cells. In addition, using siRNA treatment, we analyzed the change of sensitivity to CDDP. By knockdown of YAP using siRNA, sensitivity of CDDP was significantly increased. We concluded that YAP overexpression is correlated with CDDP resistance.
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