Vulnerable effect of anesthetics on developing brain and vascular permeability
| Project/Area Number |
25463145
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Showa University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
NISHIMURA Akiko 昭和大学, 歯学部, 助教 (00551227)
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| Research Collaborator |
USHIYAMA Akira 国立保健医療科学院, 上席主任研究官 (60291118)
AKIMOTO Yoshihiro 杏林大学, 医学部, 教授 (60184115)
KATAOKA Hanae 昭和大学, 大学院歯学研究科, 学生
OCHI Hideyuki 昭和大学, 大学院歯学研究科, 学生
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | glycocalyx / permeability / lectin / intravital scopy / proteoglycan / LPS / sepsis / glycosaminoglycan / dextran / グリコカリックス層 / 敗血症モデル / 全身麻酔薬 / 背側皮膚透明窓 / 蛍光レクチン / グリコカリックス / 敗血症 / 血管透過性 / FITC-WG / DLS / レクチン / ロダミン |
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| Outline of Final Research Achievements |
Vulnerable effect of anesthetics on developing brain is a major concern of anesthesiologist. We have approached this subject from a point of view of vascular permeability. We have accomplished a model of intravital image of the glycocalyx in mice. Glycocalyx was visualized by labelling lectin under fluorescent microscope. This layer was fragile constitutes, which was easily disrupted by LPS (lipopolysaccharide). Unltrastructural image analysis has also confirmed the layer as glycocalyx, and this positive charged layer was denuded by LPS. Its function was also analysed by using labelled dextran. We have observed the macromolecular leakage after disruption of glycocalyx layer. Our model is feasible to observe real-time alteration of vascular permeability. This model could enable to investigate how glycocalyx can be restored and regenerated once it was damaged. We offer our model to develop the pharmacoprotection of pathological sequence of damaged vessel.
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Report
(4 results)
Research Products
(6 results)
