| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Poly(ADP-ribosyl)ation is known to be involved in a variety of cellular processes by poly(ADP-ribose) polymerase (PARP). PARP involvement in MSCs (Mesenchymal Stem Cells) differentiation has been reported suggesting possible side effects of PARP inhibitors on MSCs. Therefore, effects of PARP inhibitors on MSCs were examined in this study. MSCs showed suppressed osteogenic differentiation after 1 μM PJ34 treatment without showing cytotoxicity, while differentiation of MSCs into chondrocyte or adipocyte was not affected. The mRNA induction of osteogenic markers such as and regulators of transcription factors was suppressed by PJ34 treatment. Extracellular mineralized matrix formation was also suppressed. These results strongly suggest that PARP inhibitors would suppress osteogenic differentiation and poly(ADP-ribosyl)ation may play a physiological role in osteogenic differentiation by regulating BMP-2 signaling pathway.
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