Cell polarity and mechanical response in chondrocytes.

Research Project

Project/Area Number	25463186
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Orthodontics/Pediatric dentistry
Research Institution	Kyushu University
Principal Investigator	YASUNAGA Atsushi 九州大学, 歯学研究科(研究院), 研究員 (80515990)
Co-Investigator(Kenkyū-buntansha)	TAKAHASHI Ichiro 九州大学, 歯学研究院, 教授 (70241643) HOSHI Kenji 九州大学, 歯学研究院, 助教 (90569964) TERAO Fumie 九州大学, 歯学研究院, 助教 (10510018)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000) Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	軟骨細胞 / メカニカルストレス
Outline of Final Research Achievements	We investigated the time course changes of F-actin distribution and nuclear translocation of phosphorylated extracellular signaling regulated kinase (p-ERK) 1/2 under mechanical stimulation on differentiating ATDC5 cells cultured in 3-D collagen gel culture. Nuclear translocation of p-ERK1/2 was detected 1 hour after mechanical stimulation and disappeared after 12 hours at 10% and 20% stretching. In many cells exposed to stretching force for 1 hour, stress fibers were not detected, though stress fibers were observed in the most cells and align with stretching direction under the stretch condition for 12 hour. These data indicated that mechanical stress is deeply related to actin distribution during chondrogenic differentiation.