The metagenome analysis of salivary microflora in Japanese children with Early Childhood Caries and reassessment of causative bacteria and risk factors

• Project/Area Number: 25463189
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Nagasaki University
• Principal Investigator: SATOH Kyoko 長崎大学, 病院(歯学系), 助教 (70404499)
• Co-Investigator(Kenkyū-buntansha): HOSHINO Tomonori 長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (00359960) ; SAITOH Kan 東北大学, 大学病院, 講師 (40380852) ; NISHIGUCHI Miyuki 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (10253676) ; FUJIWARA Taku 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (00228975)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: Early Childhood Caries / 唾液中細菌叢の解析 / メタゲノム解析

Outline of Final Research Achievements

We analyzed the salivary flora of children with Early Childhood Caries to reassess the causative bacteria and risk factors of Early Childhood Caries. Streptococcus mutans was not detected from two caries affected children, and Lactic acid bacteria such as the genus Lactococcus, Enterococcus, Lactbacillus, Bifidobacterium and Pediococcus were not detected in caries non affected and caries affected children.　As the results of metagenome analysis of salivary microflora, the average detection ratio of the genus Streptococcus, Abiotrophia, Neisseria, Veillonella, Rothia, Lautropia, Actinomyces, Prevotella, Gemalla and Porphyromonas in caries active children would differ from that of caries free children.
In the microorganism community analysis, it was suggested that the composition of salivary bacterial flora would be changed by having caries or not.

Research Products

• [Presentation] The metagenome analysis of salivary microflora in Japanese children with early childhood caries (2015)
  • Author(s): Fujiwara T, Satoh K, Hoshino T
  • Organizer: The 25th congress oh the International Association of Pediatric Dentistry
  • Place of Presentation: Glasgow (England)
  • Year and Date: 2015-07-01
  • Int'l Joint Research
• [Presentation] Early Childhood Caries小児の唾液中細菌叢のメタゲノム群集解析 (2015)
  • Author(s): 佐藤恭子，釜崎陽子，星野倫範，藤原 卓
  • Organizer: 第62回小児保険協会学術集会
  • Place of Presentation: 長崎ブリックホール (長崎県長崎市)
  • Year and Date: 2015-06-18
• [Presentation] Comparison of salivary microorganism community among caries affected and non-affected Japanese children by metagenome-sequencing analysis (2014)
  • Author(s): Satoh K, Hoshino T, Fujiwara T
  • Organizer: The 9th Biennial Conference of Pediatric Dentistry Association Asia
  • Place of Presentation: Singapore(Singapore)
  • Year and Date: 2014-08-22 – 2014-08-24