| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
This study aimed to identify the genetic causes of the lacking M3s in EL mice, which have 100% incidence of M3 agenesis. M3 tooth germs from EL and control mice on postnatal day 3 were dissected out and total RNA was extracted. mRNA expressional analysis was carried out using DNA microarray, real-time polymerase chain reaction and in-situ hybridization. Results: DNA microarray analysis revealed significantly decreased expression of Lef1, Fgf20 and Fgf4 in the M3s of EL mice at the bud stage relative to control mice, which was supported with both reverse-transcriptase polymerase chain reaction and quantitative real-time polymerase chain reaction analyses. Furthermore, in-situ hybridization revealed low mRNA expression levels of Fgf20 and Fgf4 in the M3s of EL mice, whereas strong signals were observed in control mice. Our results suggest that a decrease of Lef1, Fgf20 and Fgf4 expression may lead to M3 agenesis in EL mice.
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