| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
We studied the mechanism of inhibition of inflammatory response and examination of appropriate culture conditions in dental pulp cells that are deeply involved in repair and regeneration of connective tissue.
After treatment of LPS and heat stress (41℃15 min) to deciduous dental pulp cells, cell proliferations and gene expressions were investigated. In addition, cell morphology change and gene expressions were examined in deciduous dental pulp cells cultured with serum-free medium.Cell proliferation temporarily decreased due to LPS and heat stress, but recovery was observed after 72 hours. Increased IL-6 and IL-12 was observed in PT-PCR, and increased expression of HSP70 was also observed. Cells cultured with serum-free medium showed variation in cell morphology and gene expression.
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