Analysis of Th17 cells migration and activation in periodontally diseased tissues

• Project/Area Number: 25463219
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Periodontology
• Research Institution: The University of Tokushima
• Principal Investigator: HOSOKAWA Yoshitaka 徳島大学, 大学院医歯薬学研究部, 助教 (90346601)
• Co-Investigator(Kenkyū-buntansha): NAKANISHI Tadashi 徳島大学, 大学院医歯薬学研究部, 准教授 (00217770)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 歯周炎 / Th17細胞 / CCL20 / IL-6 / IL-22 / 歯肉線維芽細胞 / 歯根膜由来細胞 / ヒト歯根膜由来細胞 / Th1細胞 / CXCL10 / 歯根膜細胞 / Ｔｈ１７細胞 / ＣＣＬ２０

Outline of Final Research Achievements

We examined the effect of IL-6 and IL-22 on CCL20 production from IL-1beta-stimulated human periodontal ligament cells (HPDLC) or human gingival fibroblasts (HGFs)　in this study. We found that IL-6 and IL-22 could synergistically increase CCL20 production from IL-1beta-treated HPDLC or HGFs, respectively. Therefore, IL-6 and IL-22 are related to Th17 cells accumulation in periodontally diseased tissues to induce CCL20 production in periodontal resident cells. These results might explain that IL-6 and IL-22 are involved in the destruction of alveolar bone in periodontally diseased tissue.

Research Products

• [Journal Article] IL-4 modulates CCL11 and CCL20 productions from IL-1β-stimulated human periodontal ligament cells (2016)
  • Author(s): Yoshitaka Hosokawa, Ikuko Hosokawa, Satoru Shindo, Kazumi Ozaki, Takashi Matsuo
  • Journal Title: Cellular Physiology and Biochemistry Volume: 38 Issue: 1 Pages: 153-159
  • DOI: 10.1159/000438617
  • NAID: 120006550808
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Calcitriol supressed inflammatory reactions in IL-1β-stimulated human periodontal ligament cells (2015)
  • Author(s): Yoshitaka Hosokawa, Ikuko Hosokawa, Satoru Shindo, Kazumi Ozaki, Takashi Matsuo
  • Journal Title: Inflammation Volume: 38 Issue: 6 Pages: 2252-2258
  • DOI: 10.1007/s10753-015-0209-y
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Catechins inhibit vascular endothelial growth factor production and cyclooxygenase-2 expression in human dental pulp cells. (2015)
  • Author(s): T. Nakanishi, K. Mukai, Y. Hosokawa, D. Takegawa, T. Matsuo
  • Journal Title: International Endodontic Journal Volume: 48 Issue: 3 Pages: 277-282
  • DOI: 10.1111/iej.12312
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] IL-6 trans-signaling enhances CCL20 production from IL-1・-stimulated human periodontal ligament cells (2014)
  • Author(s): Hosokawa Y, Shindo S, Hosokawa I, Ozaki K, Matsuo T
  • Journal Title: Inflammation Volume: 37(2) Issue: 2 Pages: 381-386
  • DOI: 10.1007/s10753-013-9750-8
  • Peer Reviewed
• [Journal Article] IL-22 Enhances CCL20 Production in IL-1β-Stimulated Human Gingival Fibroblasts. (2014)
  • Author(s): Hosokawa Y, Hosokawa I, Shindo S, Ozaki K, Matsuo T.
  • Journal Title: Inflammation Volume: 37 Issue: 6 Pages: 2062-2066
  • DOI: 10.1007/s10753-014-9939-5
  • Peer Reviewed
• [Journal Article] Genipin inhibits MMP-1 and MMP-3 release from TNF-α-stimulated human periodontal ligament cells. (2014)
  • Author(s): Shindo S, Hosokawa Y, Hosokawa I, Ozaki K, Matsuo T.
  • Journal Title: Biochimie Volume: 107 Pages: 391-395
  • DOI: 10.1016/j.biochi.2014.10.008
  • Peer Reviewed
• [Journal Article] Genipin inhibits IL-1・-induced CCL20 and IL-6 production from human periodontal ligament cells (2014)
  • Author(s): Shindo S, Hosokawa Y, Hosokawa I, Ozaki K, Matsuo T
  • Journal Title: Cell Physiol Biochem Volume: 33 Issue: 2 Pages: 357-364
  • DOI: 10.1159/000356675
  • Peer Reviewed
• [Journal Article] (-)-Epigallocatechin-3-gallate inhibits CC chemokine ligand 11 production in human gingival fibroblasts (2013)
  • Author(s): Hosokawa Y, Hosokawa I, Shindo S, Ozaki K, Matsuo T
  • Journal Title: Cell Physiol Biochem Volume: 31 Issue: 6 Pages: 960-967
  • DOI: 10.1159/000350114
  • Peer Reviewed
• [Journal Article] TLR3 agonist enhances CC chemokine ligand 20 production in IL-1β-stimulated human gingival fibroblasts. (2013)
  • Author(s): Hosokawa Y, Hosokawa I, Shindo S, Ozaki K, Matsuo T.
  • Journal Title: Cellular Immunology Volume: 283 Issue: 1-2 Pages: 8-11
  • DOI: 10.1016/j.cellimm.2013.05.005
  • Peer Reviewed
• [Presentation] Alkanninはヒト歯根膜由来細胞のCCR5 ligand産生を抑制する (2015)
  • Author(s): 細川 義隆、細川 育子、進藤 智、尾崎 和美、松尾 敬志
  • Organizer: 第１４３回秋季日本歯科保存学会学術大会
  • Place of Presentation: 文京シビックホール(東京都文京区）
  • Year and Date: 2015-11-12
• [Presentation] IL-4がヒト歯根膜由来細胞のCCL11およびCCL20産生に及ぼす影響 (2015)
  • Author(s): 細川 義隆、細川 育子、進藤 智、尾崎 和美、松尾 敬志
  • Organizer: 第５８回秋季日本歯周病学会学術大会
  • Place of Presentation: アクトシティ浜松（静岡県浜松市）
  • Year and Date: 2015-09-12
• [Presentation] 歯周炎病変局所へのリンパ球浸潤機構の解析 (2015)
  • Author(s): 細川 義隆
  • Organizer: 第５８回春季日本歯周病学会学術大会
  • Place of Presentation: 幕張メッセ（千葉県船橋市）
  • Year and Date: 2015-05-15
  • Invited
• [Presentation] The Effect of Genipin on Dendritic Cells Activation (2015)
  • Author(s): Satoru Shindo, Ikuko Hosokawa, Yoshitaka Hosokawa, Kazumi Ozaki, Takashi Matsuo
  • Organizer: 93rd General Session & Exhibition of the IADR
  • Place of Presentation: Hynes Convention Center(Boston, USA)
  • Year and Date: 2015-03-13
• [Presentation] IL-4 modulates chemokine productions from IL-1β-stimulated human periodontal ligament cells (2015)
  • Author(s): Ｙｏｓｈｉｔａｋａ Ｈｏｓｏｋａｗａ, Ikuko Hosokawa, Satoru Shindo, Kazumi Ozaki, Takashi Matsuo
  • Organizer: 93rd General Session & Exhibition of the IADR
  • Place of Presentation: Hynes Convention Center(Boston, USA)
  • Year and Date: 2015-03-12
• [Presentation] Melatonin inhibits inflammatory mediators productions in human periodontal ligament cells (2015)
  • Author(s): Ikuko Hosokawa, Yoshitaka Hosokawa, Satoru Shindo, Kazumi Ozaki, Takashi Matsuo
  • Organizer: 93rd General Session & Exhibition of the IADR
  • Place of Presentation: Hynes Convention Center(Boston, USA)
  • Year and Date: 2015-03-12
• [Presentation] Shikoninがヒト歯根膜由来細胞のIL-6およびIL-8産生に与える影響 (2014)
  • Author(s): 進藤 智、細川 義隆、細川 育子、尾崎 和美、松尾 敬志
  • Organizer: 第１４１回秋季日本歯科保存学会学術大会
  • Place of Presentation: 山形テルサ（山形県山形市）
  • Year and Date: 2014-10-30
• [Presentation] 活性型ビタミンDはIL-1β刺激ヒト歯根膜細胞の炎症性サイトカイン産生を抑制する (2014)
  • Author(s): 細川 義隆、細川 育子、進藤 智、尾崎 和美、松尾 敬志
  • Organizer: 第１４０回春季日本歯科保存学会学術大会
  • Place of Presentation: 滋賀県立芸術劇場びわ湖ホール(滋賀県大津市)
  • Year and Date: 2014-06-19
• [Presentation] GenipinはE.coli LPSが誘導するヒト歯根膜細胞のIL-6およびIL-8産生を抑制する (2014)
  • Author(s): 進藤 智、細川 義隆、細川 育子、尾崎 和美、松尾 敬志
  • Organizer: 第１４０回春季日本歯科保存学会学術大会
  • Place of Presentation: 滋賀県立芸術劇場びわ湖ホール(滋賀県大津市)
  • Year and Date: 2014-06-19
• [Presentation] GenipinはTNF-αが誘導するヒト歯根膜細胞のIL-6産生を抑制する (2014)
  • Author(s): 北中 祐太郎、細川 義隆、細川 育子、尾崎 和美、松尾 敬志
  • Organizer: 第５７回春季日本歯周病学会学術大会
  • Place of Presentation: 長良川国際会議場(岐阜県岐阜市)
  • Year and Date: 2014-05-23
• [Presentation] IL-6はIL-1betaが誘導するヒト歯根膜細胞のCCL20産生を増強する
  • Author(s): 細川義隆、細川育子、進藤智、尾崎和美、松尾敬志
  • Organizer: 第５６回春季日本歯周病学会学術大会
  • Place of Presentation: タワーホール船堀 (東京都）
• [Presentation] GenipinはIL-1betaが誘導するヒト歯根膜細胞のCCL20およびIL-6産生を抑制する
  • Author(s): 進藤智、細川義隆、細川育子、尾崎和美、松尾敬志
  • Organizer: 第１３９回秋季日本歯科保存学会学術大会
  • Place of Presentation: 秋田県総合生活文化会館 (秋田県）
• [Presentation] The effect of genipin on MMP-1 and MMP-3 expression in IL-1beta-stimulated human periodontal ligament cells
  • Author(s): Satoru Shindo, Yoshitaka Hosokawa, Ikuko Hosokawa, Kazumi Ozaki, Takashi Matsuo
  • Organizer: The 13th Joint Scientific Meeting of JSCD-KACD
  • Place of Presentation: The K-hotel & Resort（韓国）