Basic research for analysis of occlusal trauma mechanism using in vitro osteoclastogenesis assay

• Project/Area Number: 25463220
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Periodontology
• Research Institution: Nagasaki University
• Principal Investigator: UKAI Takashi 長崎大学, 病院(歯学系), 講師 (20295091)
• Co-Investigator(Kenkyū-buntansha): 原 宜興 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (60159100)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 咬合性外傷 / 破骨細胞 / TNFα / RANKL / TNFa / RANKL前刺激 / 吸収活性 / osteoclast

Outline of Final Research Achievements

RANKL pre-stimulation before stimulation of TNFa can induce active osteoclast formation. We showed the condition of RANKL pre-stimulation for forming of active osteoclasts. In this condition, osteoclast precursors expressed TRAF6. Moreover, blocking of TRAF6 at RANKL pre-stimulation inhibited TNFa inducing active osteoclast formation.
These results suggest RANKL pre-stimulation that TRAF6 is activated is important for forming of TNFa inducing active osteoclasts.

Research Products

• [Journal Article] 37.RANKL pretreatment plays an important role in the differentiation of pit-forming osteoclasts induced by TNF-α on murine bone marrow macrophages. (2015)
  • Author(s): Yamashita Y, Ukai T, Nakamura H, Yoshinaga Y, Kobayashi H, Takamori Y, Noguchi S, Yoshimura A, Hara Y
  • Journal Title: Arch Oral Biol. Volume: 60(9) Issue: 9 Pages: 1273-1282
  • DOI: 10.1016/j.archoralbio.2015.06.002
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 破骨細胞前駆細胞へのRANKL前刺激はTNFαによる吸収能をもった破骨細胞分化に重要である (2016)
  • Author(s): 鵜飼 孝、山下恭徳、原 宜興
  • Organizer: 第２３回日本歯科医学会総会
  • Place of Presentation: 福岡国際会議場（福岡県・福岡市）
  • Year and Date: 2016-10-21
• [Presentation] RANKL前刺激を受けた破骨細胞前駆細胞はTNF-αにより吸収能をもった破骨細胞に分化する (2016)
  • Author(s): 鵜飼孝, 山下恭德, 原 宜興
  • Organizer: 歯科医学会総会
  • Place of Presentation: 福岡国際会議場（福岡県・福岡市）
  • Year and Date: 2016-10-21
• [Presentation] マウス骨髄マクロファージにおいてRANKL前刺激はTNF-a刺激による吸収活性を持つ破骨細胞の分化に重要な役割をはたす (2015)
  • Author(s): 山下恭德, 鵜飼孝, 中村弘隆, 小林弘樹, 高森雄三, 野口惠司, 吉村篤利, 原 宜興
  • Organizer: 平成27年度日本歯周病学会九州五大学・日本臨床歯周病学会九州支部合同研修会
  • Place of Presentation: アクロス福岡（福岡県・福岡市）
  • Year and Date: 2015-11-08
• [Presentation] Vascular endothelial cells promotes bone destruction in rat occlusal trauma. (2015)
  • Author(s): Ukai T, Yamashita Y, Nakamura H, Yoshimura A, Hara Y
  • Organizer: 11th APSP meeting
  • Place of Presentation: Bali, Indonesia
  • Year and Date: 2015-10-08
  • Int'l Joint Research
• [Presentation] RANKL priming accelerates osteoclast formation with resorptive activity in TNF-alpha-stimulated murine bone marrow macrophages independent of RANKL (2014)
  • Author(s): Yamashita Y, Ukai T, Takamori Y, Noguchi S, Montenegro Raudales JL, Nakamura H, Ozaki Y, Shiraishi C, Yoshimura A, Hara Y
  • Organizer: 100th American Academy of Periodontology
  • Place of Presentation: San Francisco convention center (USA)
  • Year and Date: 2014-09-19 – 2014-09-24
• [Presentation] RANKL前刺激を受けた破骨細胞前駆細胞はTNF-α 刺激によりRANKL非依存的に骨 吸収活性をもった破骨細胞形成を形成する (2014)
  • Author(s): 山下恭徳, 鵜飼 孝
  • Organizer: 第35回日本炎症・再生医学会・第1回日本骨免疫会議
  • Place of Presentation: 万国津梁館（名護市）
  • Year and Date: 2014-07-01 – 2014-07-04
• [Presentation] TNF-α刺激により破骨細胞形成が促進されるRANKL前刺激条件の検討 (2013)
  • Author(s): 山下恭德, 鵜飼 孝, 吉村篤利, 金子高士, 白石千秋, 吉永美穂, 吉永泰周, 原 宜興
  • Organizer: 日本歯科保存学会2013年度秋季学術大会
  • Place of Presentation: 秋田県総合生活文化会館（秋田市）
• [Presentation] TNFα刺激により破骨細胞形成が促進されるRANKL前刺激条件の検討 (2013)
  • Author(s): 山下 恭德、鵜飼 孝、原 宜興
  • Organizer: 平成25年度日本歯周病学会九州五大学・日本臨床歯周病学会九州支部合同研修会
  • Place of Presentation: 九州歯科大学（北九州市）