The functional analysis of SOD2, antioxidantdefense in periodontal tissue
| Project/Area Number |
25463221
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Periodontology
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHIKO SHIMIZU 千葉大学, 医学科研究院, 准教授 (40301791)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 酸化ストレス / セメント質 / ＳＯＤ２ / SOD2 / セメント芽細胞 / セメント質肥大 / 歯周組織 |
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| Outline of Final Research Achievements |
SOD (Superoxide dismutase) is an enzyme that alternately catalyzes the dismutation of the superoxide (O2－) radical into either ordinary molecular oxygen (O2) or hydrogen peroxide (H2O2). Thus, SOD is an important antioxidantdefense in nearly all living cells exposed to oxygen. We generated DMP1 cre SOD2 KO mice and conditionally inactivated SOD2 in osteocytes and odontoblasts. We found that loss of SOD2 enhanced thickness of cementum in teeth. In the other hands, periodontal ligament and alveolar bone in DMP1 cre SOD2 KO mice were normal compared with wild type mice. Overexpression of SOD2 in cementoblasts decreased the expression of DMP1 and CP23, which were differentiation markers of cementum. On the contrary, knockdown of SOD2 using siRNA increased both proliferation and differentiation in cementoblasts. These data suggested that SOD2 supressed proliferation and differentiation in cementoblasts.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Donepezil prevents RANK-induced bone loss via inhibition of osteoclast differentiation by downregulating acrtylcholinesterase.2015
Author(s)
Sato T, Enoki Y, Sakamoto Y, Yokota K, Okubo M, Matsumoto M, Hayashi N, Usui M, Kokabu S, Miura T, Nakazato Y, Araki N, Fukuda T, Okazaki Y, Suda T, Takeda S, Yoda T
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Netrin-4 derived from murine vascular endothelial cells inhibits osteoclast differentiation in vitro and prevents bone loss in vivo.2014
Author(s)
Enoki Y, Sato T, Tanaka S, Iwata T, Usui M, Takeda S, Kokabu S, Matsumoto M, Okubo M, Nakashima K, Yamato M, Okano T, Fukuda T, Chida D, Imai Y, Yasuda H, Nishihara T, Akita M, Oda H, Okazaki Y, Suda T, Yoda T.
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Journal Title
FEBS Lett.
Volume: 588
Pages: 2262-2269
Related Report
Peer Reviewed
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