| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
It is shown that the early destruction feature of periodontal ligament (PDL) on periodontal disease is the degradation of the non-collagenous proteins (NCP) covered collagen bundles. The aim is the characterization of 110kDa Stains-all positive protein (SAPP) belonged to NCP, which is degraded first by the neutrophil elastase increased in the inflamed PDL.
The 110kDa SAPP was detected by Stains-all staining after SDS electrophoresis in the NCP extracted from porcine mandibles' deciduous incisor tooth PDL and identified to be decorin by MASS and immunochemical determination. The decorin purified by heparin affinity column was determined to bind the TGF-b1 by the cell culture system of hPDL cell and ELISA. On the periodontal disease, it is suggested that the degradation of decorin bound to the collagen bundles by the action of the neutrophil elastase releases the TGF-b 1 to reconstruct the injured PDL.
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