| Project/Area Number |
25463236
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Social dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
Takehara Sachiko 東京医科歯科大学, 統合国際機構, 特任助教 (60622438)
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| Co-Investigator(Renkei-kenkyūsha) |
YANAGISHITA Masaki 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (70132793)
INOUE KA 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (90302877)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 唾液タンパク質 / ムチン / 糖鎖 / 抗菌作用 / 誤嚥性肺炎 / 口腔内細菌 / 細菌叢解析 / マーモセット / 唾液 / MUC7 / 細菌 / 分解 / 唾液糖タンパク質 / 唾液ムチン / 糖鎖修飾 |
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| Outline of Final Research Achievements |
General degradation patterns of salivary proteins and glycoproteins were examined by SDS-polyacrylamide-gel-electrophoresis. A protein band was identified as MUC7 by Western blot analysis using an antibody recognizing an N-terminal epitope. The MUC7 signal disappeared rapidly after 20-minutes of incubation. In contrast, the band of MUC7 stained for its carbohydrate components remained visible near its original position for a longer time indicating that the rapid loss of Western blot signal was due to the specific removal of the N-termimal epitope. MUC7 was highly susceptible to specific proteolysis in saliva, though major part of MUC5B was more resistant to degradation. The N-terminal region of MUC7, particularly sensitive to proteolytic degradation, has also been proposed to have distinct biological function such as antibacterial activities. Quick removal of this region may have biologically important implication.
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