Exploration of relevant factors of oral microbiome maturation by using molecular approach

• Project/Area Number: 25463249
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Social dentistry
• Research Institution: Kyushu University
• Principal Investigator: Takeshita Toru 九州大学, 歯学研究科(研究院), 准教授 (50546471)
• Co-Investigator(Kenkyū-buntansha): YAMASHITA Yoshihisa 九州大学, 大学院歯学研究院, 教授 (20192403)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 口腔マイクロバイオーム / 16S rRNA / 舌苔 / 細菌 / マイクロバイオーム / 口腔 / 常在フローラ / 次世代シーケンサー / 成熟

Outline of Final Research Achievements

This study revealed that compositional shift in tongue microbiome occurs during two years from birth. In particular, the relative abundances of dominant bacterial genera greatly changed after introduction of baby food (about 200 days from birth) in many of the subjects, implying that diet change would be a trigger of bacterial community succession. Our result would be the important basis for the establishment of a new approach for oral health promotion, which aims to induce healthy oral microbiome.

Research Products

• [Journal Article] Bacterial diversity in saliva and oral health-related conditions: the Hisayama Study. (2016)
  • Author(s): Takeshita T, Kageyama S, Furuta M, Tsuboi H, Takeuchi K, Shibata Y, Shimazaki Y, Akifusa S, Ninomiya T, Kiyohara Y, Yamashita Y.
  • Journal Title: Science Reorts Volume: 6 Issue: 1 Pages: 1-11
  • DOI: 10.1038/srep22164
  • Peer Reviewed / Open Access
• [Journal Article] 口腔常在微生物叢の構成と健康との関連 (2016)
  • Author(s): 竹下徹、山下喜久
  • Journal Title: 日本乳酸菌学会誌 Volume: 27 Pages: 3-9
  • NAID: 130005628246
  • Peer Reviewed
• [Journal Article] Acetaldehyde production by major oral microbes (2015)
  • Author(s): Moritani K Takeshita T, Shibata Y, Ninomiya T, Kiyohara Y, Yamashita Y.
  • Journal Title: Oral Diseases Volume: 21 Issue: 6 Pages: 748-754
  • DOI: 10.1111/odi.12341
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 口腔マイクロバイオームと疾患との関連 (2015)
  • Author(s): 山下喜久、竹下徹
  • Journal Title: 臨床と微生物 Volume: 42 Pages: 711-715
• [Journal Article] 口腔マイクロバイオーム評価の臨床的意義 (2015)
  • Author(s): 山下喜久、竹下徹
  • Journal Title: 臨床化学 Volume: 44 Pages: 290-297
• [Journal Article] Mechanical induction of interleukin-11 regulates osteo/cementoblastic differentiation of human periodontal ligament stem/progenitor cells. (2015)
  • Author(s): Monnouchi S, Maeda H, Yuda A, Hamano S, Wada N, Tomokiyo A, Koori K, Sugii H, Serita S, Akamine A
  • Journal Title: J Periodontal Res Volume: 50 Issue: 8 Pages: 231-239
  • DOI: 10.1111/iej.12361
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Dental plaque development on a hydroxyapatite disk in young adults observed by using a barcoded pyrosequencing approach (2015)
  • Author(s): Takeshita T, Yasui M, Shibata Y, Furuta M, Saeki Y, Eshima N, Yamashita Y
  • Journal Title: Sci. Rep. Volume: 5 Pages: 8136-8136
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Distinct composition of the oral indigenous microbiota in South Korean and Japanese adults (2014)
  • Author(s): Takeshita T, Matsuo K, Furuta M, Shibata Y, Fukami K, Shimazaki Y, Akifusa S, Han DH, Kim HD, Yokoyama T, Ninomiya T, Kiyohara Y, Yamashita Y
  • Journal Title: Sci Rep Volume: 4 Issue: 1 Pages: 6990-6990
  • DOI: 10.1038/srep06990
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification of the microbiota in carious dentin lesions using 16S rRNA gene sequencing (2014)
  • Author(s): Obata J, Takeshita T, Shibata Y, Yamanaka W, Unemori M, Akamine A, Yamashita Y
  • Journal Title: PLoS One Volume: 9 Issue: 8 Pages: e103712-e103712
  • DOI: 10.1371/journal.pone.0103712
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Supervised machine learning-based classification of oral malodor based on the microbiota in saliva samples. (2014)
  • Author(s): Nakano Y, Takeshita T, Kamio N, Shiota S, Shibata Y, Suzuki N, Yoneda M, Hirofuji T, Yamashita Y.
  • Journal Title: Artificial Intelligence in Medicine Volume: 60 Issue: 2 Pages: 97-101
  • DOI: 10.1016/j.artmed.2013.12.001
  • Peer Reviewed / Open Access
• [Presentation] 疾患および健康に関連する口腔常在マイクロバイオームの探索 (2015)
  • Author(s): 竹下徹
  • Organizer: 日本乳酸菌学会秋季セミナー
  • Place of Presentation: 東京都
  • Year and Date: 2015-11-27
  • Invited
• [Presentation] Salivary microbiome and environmental conditions in the oral cavity (2015)
  • Author(s): Takeshita T, Yamashita Y
  • Organizer: 第63回国際歯科研究学会(JADR)
  • Place of Presentation: 福岡
  • Year and Date: 2015-10-31
  • Invited
• [Presentation] A Molecular epidemiology study exploring oral microbiome structure associated with human health (2015)
  • Author(s): Takeshita T, Yamashita Y
  • Organizer: 歯科基礎医学会サテライトシンポジウム
  • Place of Presentation: 新潟
  • Year and Date: 2015-09-11
• [Presentation] Bacterial diversity and community types in saliva and oral environment (2015)
  • Author(s): Toru Takeshita, Michiko Furuta, Hidenori Tsuboi, Shinya Kageyama, Yoshihiro Shimazaki, Toshiharu Ninomiya, Yutaka Kiyohara, Yoshihisa Yamashita
  • Organizer: 5th International Human Microbiome Congress
  • Place of Presentation: Luxembourg
  • Year and Date: 2015-04-01
  • Int'l Joint Research
• [Presentation] Development of oral indigenous microbiota in early childhood (2014)
  • Author(s): Takeshita T
  • Organizer: ISME15
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2014-08-25 – 2014-08-29
• [Presentation] 乳幼児期における口腔常在フローラの成熟過程 (2013)
  • Author(s): 竹下徹
  • Organizer: 九州口腔衛生学会
  • Place of Presentation: 福岡市