| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
A total of 254 staphylococcal isolates were collected from oral cavity of 526 healthy children were analysed. Multiplex PCR was performed to detect mecA, PVL genes and ACME-arcA gene. SCCmec typing was determined for all isolates, and coagulase gene (coa) genotype was assigned to all S. aureus. ST (sequence type) based on MLST scheme, agr type, prevalence of virulence factors, antibiotic susceptibility were analysed for the selected 20 S. aureus isolates.
The most prevalent species was S. aureus (n=147), followed by S. epidermidis (n=89), S. warneri (n=12), S. haemolyticus (n=4), and S. hominis (n=2). The mecA was detected in 6.1% of S. aureus, 60.7% of S. epidermidis, and 8.3% in S. warneri, and SCCmec was typed as IV (75%) or V, with IVa subtype being dominant. MRSA were classified into ST1, ST5, ST8, ST89, ST120. Two ST8 were SCCmec IVl, coa-IIIa, and had sec, sei, sel, tst-1, and spj/sasL gene which is characteristic trait for an emerging Japanese MRSA clone.
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