Studies on the adherence factors of mutans streptococci to human oral cavity by comparative investigation among primates

• Project/Area Number: 25463270
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Social dentistry
• Research Institution: Tsurumi University
• Principal Investigator: Imai Susumu 鶴見大学, 歯学部, 非常勤講師 (80072958)
• Co-Investigator(Kenkyū-buntansha): HANADA Nobuhiro 鶴見大学, 歯学部, 教授 (70180916) ; NOMURA Yoshiaki 鶴見大学, 歯学部, 准教授 (90350587) ; OOTA Kazumasa 東京医科大学, 医学部, 准教授 (30307376)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ミュータンスレンサ球菌 / バイオフィルム / S. mutans / S. sobrinus / S. troglodyte / S. dentirousetti / う蝕 / グルコシルトランスフェラーゼ / バイオフィルム形成 / S. troglodytae / Streptococcus mutans / Streptococcus sobrinus / 付着因子 / 唾液 / チンパンジー / 定着因子

Outline of Final Research Achievements

Adherence factors of cariogenic bacteria, mutans streptococci, to human oral cavity were investigated by comparison among animals including humans. Streptococcus troglodytae and Streptococcus dentirousetti derived from chimpanzee formed biofilm on enamel slabs, decreased pH values underneath the biofilm and demineralized enamel like S. mutans and S. sobrinus from humans suggesting that both strains might be cariogenic. Phylogenetic analysis of glucosyltransferase (GTF) genes of S. troglodytae and S. dentirousetti showed that both strains possessed GTF genes as adherence factors similar to S. mutans and S. sobrinus, respectively.

Research Products

• [Journal Article] Opportunistic Bacteria in Tonsil and Dental Plaque are Indicator for Oral Care. (2016)
  • Author(s): Chiyoko Hakuta, Ayako Okada, Kaoru Sogabe, Erika Kakuta, Keiko Endo, Susumu Imai, Masaaki Okamoto, Yoshiaki Nomura and Nobuhiro Hanada
  • Journal Title: J Oral Craniofac Sci Volume: 2 Pages: 33-34
  • Peer Reviewed
• [Journal Article] Evaluation of antibacterial activity of visible light-responsive TiO2-based photocatalyst coating on orthodontic materials against cariogenic bacteria (2016)
  • Author(s): Tomomi Sudo, Susumu Imai, Tsuyoshi Ochiai, Hiroyuki Kanzaki, Yusuke Morita, Nobuhiro Hanada, Yoshiki Nakamura
  • Journal Title: Asian Pacific Journal of Dentistry Volume: 16 Pages: 15-22
  • Peer Reviewed
• [Journal Article] Screening of Probiotic Candidates in Human Oral Bacteria for the Prevention of Dental Disease (2015)
  • Author(s): Terai T, Okumura T, Imai S, Nakao M, Yamaji K, Ito M, Nagata T, Kaneko K, Miyazaki K, Okada A, Nomura Y, Hanada N
  • Journal Title: PLoS One Volume: 10 Issue: 6 Pages: 1-20
  • DOI: 10.1371/journal.pone.0128657
  • Peer Reviewed / Open Access
• [Journal Article] Effects of Lactobacillus crispatus as a candidate for oral probiotic bacteria on Haemophilus influenzae. (2015)
  • Author(s): Kaoru Sogabe-Ashigaki, Susumu Imai, Ayako Okada, Khairul Matin, Tomohiko Terai, Takekazu Okumura, Nobuhiro Hanada, Hiroshi Kawahara.
  • Journal Title: Asian Pacific Journal of Dentistry Volume: 15 Pages: 3-11
  • NAID: 40020570176
  • Peer Reviewed
• [Journal Article] Application of chimeric glucanase comprising mutanase and dextranase for prevention of dental biofilm formation (2015)
  • Author(s): Otsuka R, Imai S, Murata T, Nomura Y, Okamoto M, Tsumori H, Kakuta E, Hanada N, Momoi Y
  • Journal Title: Microbiol Immunol Volume: 59 Issue: 1 Pages: 28-36
  • DOI: 10.1111/1348-0421.12214
  • NAID: 40020351695
  • Peer Reviewed / Open Access
• [Journal Article] Effects of Lactobacillus crispatus as a candidate for oral probiotic bacteria on Haemophilus influenzae (2015)
  • Author(s): Kaoru Sogabe-Ashigaki, Susumu Imai, Ayako Okada, Khairul Matin, Tomohiko Terai, Takekazu Okumura, Nobuhiro Hanada, Hiroshi Kawahara
  • Journal Title: Asian Pacific Journal of Dentistry Volume: 15 Pages: 3-11
  • NAID: 40020570176
  • Peer Reviewed
• [Journal Article] リン酸化オリゴ糖カルシウムおよびフッ化物によるエナメル質再石灰化促進効果について (2015)
  • Author(s): 筒井 廉、今井 獎、花田信弘、朝田芳信
  • Journal Title: 小児歯科額雑誌 Volume: 53 Pages: 35-46
  • NAID: 130005129166
  • Peer Reviewed
• [Journal Article] Effect of silver ion coating of fi xed orthodontic retainers on the growth of oral pathogenic bacteria (2014)
  • Author(s): Yusuke MORITA, Susumu IMAI, Ai HANYUDA, Khairul MATIN, Nobuhiro HANADA and Yoshiki NAKAMURA
  • Journal Title: Dental Materials Journal Volume: 33 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Serum antibody to Porphyromonas gingivalis in metabolic syndrome among an older Japanese population (2014)
  • Author(s): Masanori Iwasaki, Kumiko Minagawa, Misuzu Sato, Noboru Kaneko, Susumu Imai, Akihiro Yoshihara and Hideo Miyazaki
  • Journal Title: Gerodontology Volume: 13 Issue: 2 Pages: 1-8
  • DOI: 10.1111/ger.12135
  • Peer Reviewed
• [Journal Article] Assessment of the Level of Periodontopathic Pathogens in Oral cavity by Mouth Air Analysis (2014)
  • Author(s): Takako Arai, Susumu Imai, Nobuhiro Hanada, Kyuichi Kamoi and Yukihiro Numabe
  • Journal Title: 日本歯周病学会誌 Volume: 56 Pages: 1-12
  • NAID: 130004842593
  • Peer Reviewed
• [Journal Article] Distributionof Streptococcus troglodytae and Streptococcus dentirousetti in chim- panzee oral cavities. (2013)
  • Author(s): Miyanohara M, Imai S, Okamoto M, Saito W, Nomura Y, Momoi Y, Tomonaga M, Hanada N.
  • Journal Title: Microbiology and Immunology, in press Volume: [Epub ahead of print] Issue: 5 Pages: 359-65
  • DOI: 10.1111/1348-0421.12047
  • Peer Reviewed
• [Journal Article] Biofilm formation on titanium and hydroxyapatiee surface using artificial mouth system. (2013)
  • Author(s): Yoshida E, Imai S, Hanada N. Hayakawa T.
  • Journal Title: J Hard Tissue Biology Volume: 22(4) Pages: 417-422
  • Peer Reviewed
• [Journal Article] A novel method for enhancement of peptide vaccination utilizing T-cell epitopes from conventional vaccines. (2013)
  • Author(s): Yano A, Miwa Y, Kanazawa Y, Ito K, Makino M, Imai S, Hanada N, Nisizawa T.
  • Journal Title: Vaccine Volume: 31(11) Issue: 11 Pages: 1510-1515
  • DOI: 10.1016/j.vaccine.2012.12.083
  • Peer Reviewed
• [Journal Article] Streptococcus troglodytae sp.nov., from the Chimpanzee Oral Cavity (2012)
  • Author(s): Okamoto M, Imai S, Miyanohara M, Saito W, Momoi Y, Abo T, Nomura Y, Ikawa T, Ogawa T, Miyabe-Nishiwaki T, Kaneko A, Watanabe A, Watanabe S, Hayashi M, Tomonaga M, Hanada N
  • Journal Title: Int J Syst Evol Microbiol Volume: (Epub ahead of print) Issue: Pt_2 Pages: 418-22
  • DOI: 10.1099/ijs.0.039388-0
  • Peer Reviewed
• [Presentation] Trial of a Liquid Carrier Type Dental Drug Delivery Tray. (2015)
  • Author(s): Ayako Okada, Takatoshi Murata Khairul Matin, Mayu Miyanohara, Kaoru Sogabe, Ryoko Otsuka, Masaru Kirihara, Masazumi Matsuzawa, Tomomi Sudo, Susumu Imai, Junji Tagami, Nobuhiro Hanada.
  • Organizer: the 63rd Annual meeting of JADR,
  • Place of Presentation: FUKUOKA INTERNATIONAL CONGRESS CENTER(Fukuoka, Japan)
  • Year and Date: 2015-10-30
  • Int'l Joint Research
• [Presentation] Biofilm formation and demineralization by mutans streptococci from humans and chimpanzees. (2015)
  • Author(s): Susumu Imai, Ayako Okada, Masaaki Okamoto, Mayu Miyanohara, Kazumasa Oota, Yoshiaki Nomura, Yasuko Momoi, Masaki Tomonaga and Nobuhiro Hanada.
  • Organizer: the 63rd Annual meeting of JADR
  • Place of Presentation: FUKUOKA INTERNATIONAL CONGRESS CENTER(Fukuoka, Japan)
  • Year and Date: 2015-10-30
  • Int'l Joint Research
• [Presentation] スポンジを用いた口腔細菌採取法の有効性について (2015)
  • Author(s): マティン カイルール, 岡田 彩子, 阿保 備子, 大塚 良子, 根深 研一, 今井 奬, 花田 信弘
  • Organizer: 64回日本口腔衛生学会・総会
  • Place of Presentation: つくば国際会議場（茨城県つくば市）
  • Year and Date: 2015-05-27
• [Presentation] Trends of Radionuclide Concentrations in Japanese Milk Teeth (2015)
  • Author(s): Kazuhiko INOUE, Ichiro YAMAGUCHI, Susumu IMAI, Takatoshi MURATA, Nobuhiro HANADA
  • Organizer: 15th International Congress of Radiation Research
  • Place of Presentation: Kyoto International Conference Center(Kyoto, Japan)
  • Year and Date: 2015-05-25
  • Int'l Joint Research
• [Presentation] Cytotoxicity of Reactive Oxygen Species Based Disinfectants on Epithelial Cell, (2015)
  • Author(s): A. Okada, K. Matin, S. Imai, T. Kikuchi and N. Hanada
  • Organizer: 93rd General Session & Exhibition of the IADR
  • Place of Presentation: John B. Hynes Veterans Memorial Convention Center, Boston, USA
  • Year and Date: 2015-03-11 – 2015-03-14
• [Presentation] Antibacterial Effects of a Flavored Superoxidized Water on Oral Pathogens (2015)
  • Author(s): K. Matin, A. Okada, K. Nebuka, S. Imai, N. Hanada and J. Tagami
  • Organizer: 93rd General Session & Exhibition of the IADR
  • Place of Presentation: John B. Hynes Veterans Memorial Convention Center, Boston, USA
  • Year and Date: 2015-03-11 – 2015-03-14
• [Presentation] Effect of phosphoryl oligosaccharide calcium and fluorides on remineralization of enamel (2014)
  • Author(s): Yasushi Tsutsui, Susumu Imai, Nobuhiro Hanada and Yoshinobu Asada
  • Organizer: 第62回JADR総会・学術大会
  • Place of Presentation: 大阪府 大阪市 KKRホテル大阪
  • Year and Date: 2014-12-03 – 2014-12-05
• [Presentation] Probiotic Effects of Lactobacillus crispatus YIT 12319 on Haemophilus influenzae Associated with Chronic Obstructive Pulmonary Disease (2014)
  • Author(s): Kaoru Ashigaki, Susumu Imai, Gaku Akimaru, Ayako Okada, Takekazu Okumura, Tomohiko Terai, Kazuaki Yamaji, Nobuhiro Hanada and Hiroshi Kawahara
  • Organizer: 第62回JADR総会・学術大会
  • Place of Presentation: 大阪府 大阪市 KKRホテル大阪
  • Year and Date: 2014-12-03 – 2014-12-05
• [Presentation] Antibacterial effect of photocatalytic titanium oxide responsive to visible light (2014)
  • Author(s): Tomomi SUDO, Susumu IMAI, Tsuyoshi OCHIAI, Nobuhiro HANADA, Yoshiki NAKAMURA
  • Organizer: 第62回JADR総会・学術大会
  • Place of Presentation: 大阪府 大阪市 KKRホテル大阪
  • Year and Date: 2014-12-03 – 2014-12-05
• [Presentation] Application of Chimeric Glucanase of Mutanase and Dextranase for the Prevention of Dental Biofilm Formation (2014)
  • Author(s): R. Otsuka, S. Imai, T. Murata, Y. Nomura, M. Okamoto, H. Tsumori, M. Miyanohara, A. Okada, E. Kakuta, N. Hanada, Y. Momoi
  • Organizer: 第62回JADR総会・学術大会
  • Place of Presentation: 大阪府 大阪市 KKRホテル大阪
  • Year and Date: 2014-12-03 – 2014-12-05
• [Presentation] ミュ－タンスレンサ球菌及びLactobacillus gasseriの相互作用について (2014)
  • Author(s): 菊地朋宏、今井 奬、岡田彩子、奥村剛一、花田信弘
  • Organizer: 第63回日本口腔衛生学会・総会
  • Place of Presentation: 熊本県 熊本市 国際交流会館
  • Year and Date: 2014-05-29 – 2014-05-31
• [Presentation] 活性酸素種のHeLa-KB細胞に対する細胞毒性の検討 (2013)
  • Author(s): 岡田彩子、今井奨、菊地朋宏、花田信弘
  • Organizer: 第62回日本口腔衛生学会・総会
  • Place of Presentation: 松本市・キッセイ文化ホール（長野県）
• [Presentation] 機能性食品の機能評価方法：エナメル質脱灰性評価 (2013)
  • Author(s): 今井 奬
  • Organizer: 第62回日本口腔衛生学会・総会
  • Place of Presentation: 松本市・キッセイ文化ホール（長野県）
• [Presentation] 機能性食品の機能評価方法：口臭抑制機能評価 (2013)
  • Author(s): 今井 奬
  • Organizer: 第62回日本口腔衛生学会・総会
  • Place of Presentation: 松本市・キッセイ文化ホール（長野県）
• [Presentation] ムタナーゼとデキストラナーゼからなるキメラ酵素によるバイオフィルムの分解 (2013)
  • Author(s): 大塚 良子、角田 衣理加、野村 義明、今井 奬、津守 秀明、桃井 保子、 花田 信弘
  • Organizer: 鶴見大学歯学会第77回例会
  • Place of Presentation: 横浜市・鶴見大学会館（神奈川県）
• [Presentation] 2種次亜塩素酸電解水の細胞毒性に関する検討 (2013)
  • Author(s): 岡田彩子、マティン カイル－ル、根深研一、今井 奬、花田信弘、田上順次
  • Organizer: 第139回日本歯科保存学会2013年度秋季学術大会
  • Place of Presentation: 秋田県総合生活文化会館（秋田県）
• [Presentation] ムタナーゼとデキストラナーゼからなるキメラ酵素はバイオフィルムの形成を阻害した (2013)
  • Author(s): 大塚 良子、今井 奬、花田 信弘、桃井 保子
  • Organizer: 第139回日本歯科保存学会2013年度秋季学術大会
  • Place of Presentation: 秋田県総合生活文化会館（秋田県）