| Project/Area Number |
25502002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Reliable environmental measurement methods
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| Research Institution | Kobe University |
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Principal Investigator |
SAITO Keiitsu 神戸大学, 人間発達環境学研究科, 教授 (50162194)
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| Research Collaborator |
YAMAZAKI Shigeo 岡山理科大学, 理学部, 教授
SAKANE Genta 岡山理科大学, 理学部, 准教授
KODAMATANI Hitoshi 鹿児島大学, 大学院理工学研究科, 助教
SAGA Makoto 神戸大学, 大学院人間発達環境学研究科, 博士課程後期課程大学院生
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Keywords | 化学発光 / 蛍光 / HPLC / FIA / ルテニウム(III)錯体 / ルミノール / 銅(I)錯体 / メチルマロン酸 / ホモシステイン / ホモシスチン / 第二級アミン / グルコース / 浸透制限型イオン交換樹脂 / ルテニウム錯体 / 銅錯体 / L-ホモシステイン / 亜ジチオン酸ナトリウム / マロンジアルデヒド / メチルイミダゾール |
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| Outline of Final Research Achievements |
Measurement systems for 4-methylimidazole, homocysteine, homocystine, and methylmalonic acid using high-performance liquid chromatography (HPLC) with tris(2,2'-bipyridine)ruthenium(III) chemiluminescence detection were developed and applied to cola beverage, serum, and urine samples. Measurement systems for secondary amines and glucose using HPLC or flow injection analysis (FIA) with luminol chemiluminescence detection were developed and applied to tap water and non-carbonated soft drink samples. Copper(I) complexes with N,N'-bis(2-methylquinolyl)dimethylethylenediamine (BQEN) and N,N'-bis(2-methylquinolyl)dimethyl-1,3-propanediamine (BQPN) were found to fluoresce in aqueous media. Measurement systems for sodium dithionite and hydrazine using FIA with copper(I)-bqen or -bqpn complex fluorescence detection were developed and applied to spring water samples.
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