Regulation of retrotransposons by epigenetic modifications and molecular chaperons
| Project/Area Number |
25503003
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
epigenetics
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | エピジェネティクス / 転移因子 / 生殖細胞 / DNAメチル化 / piRNA / 熱ショックタンパク質 / レトロトランスポゾン / 小分子RNA / 減数分裂 / エピジェネティック制御 / レトロとランスポゾン / 分子シャペロン / 転写後制御 |
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| Outline of Final Research Achievements |
To elucidate the mechanisms of retrotransposon regulation during the development of male germ cells in mice, we analyzed the levels of DNA methylation and RNA expression of a large number of retrotransposons in Pld6 and Dnmt3L mutant germ cells by deep sequencing. We revealed that the post-transcriptional silencing mechanism via piRNA-dependent mRNA cleavage plays a primary role in early germ cells called prospermatogonia, whereas the transcriptional silencing mechanism via DNA methylation later becomes a primary mechanism in spermatocytes executing meiosis. We also revealed, by genetic studies on Hsp90-alpha knockout mice, that Hsp90-alpha plays an important role in the step where piRNAs become loaded onto the piRNA-dependent RNases (i.e., PIWI-family proteins) in prospermatogonia.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Chd5 Regulates MuERV-L/MERVL Expression in Mouse Embryonic Stem Cells Via H3K27me3 Modification and Histone H3.1/H3.2.2015
Author(s)
Hayashi M, Maehara K, Harada A, Semba Y, Kudo K, Takahashi H, Oki S, Meno C, Ichiyanagi K, Akashi K, Ohkawa Y.
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Journal Title
J Cell Biochem
Volume: 117
Issue: 3
Pages: 780-792
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] HSP90α plays an important role in piRNA biogenesis and retrotransposon repression in mouse2014
Author(s)
Ichiyanagi, T., Ichiyanagi, K., Ogawa, A., Kuramochi-Miyagawa, S., Nakano, T., Chuma, S., Sasaki, H.
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Journal Title
Nucleic Acids Res
Volume: 42
Issue: 19
Pages: 11903-11
DOI
Related Report
Peer Reviewed / Open Access
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