| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
To elucidate the mechanisms of retrotransposon regulation during the development of male germ cells in mice, we analyzed the levels of DNA methylation and RNA expression of a large number of retrotransposons in Pld6 and Dnmt3L mutant germ cells by deep sequencing. We revealed that the post-transcriptional silencing mechanism via piRNA-dependent mRNA cleavage plays a primary role in early germ cells called prospermatogonia, whereas the transcriptional silencing mechanism via DNA methylation later becomes a primary mechanism in spermatocytes executing meiosis. We also revealed, by genetic studies on Hsp90-alpha knockout mice, that Hsp90-alpha plays an important role in the step where piRNAs become loaded onto the piRNA-dependent RNases (i.e., PIWI-family proteins) in prospermatogonia.
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