Analyses of the microRNAs regulating the expression of type 2 diabetes mellitus-susceptibility genes

Research Project

Project/Area Number	25504007
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Integrated Nutrition Science
Research Institution	University of Shizuoka
Principal Investigator	Kobayashi Kimiko 静岡県立大学, 食品栄養科学部, 教授 (90215319)
Co-Investigator(Kenkyū-buntansha)	TOSHINAO GODA 静岡県立大学, 食品栄養科学部, 教授 (70195923)
Project Period (FY)	2013-04-01 – 2016-03-31
Project Status	Completed (Fiscal Year 2015)
Budget Amount *help	¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000) Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	生活習慣病 / 糖尿病 / microRNA / HNF1B遺伝子 / 糖尿病リスク遺伝子 / HNF1B / リスク遺伝子
Outline of Final Research Achievements	We found the 3’UTR SNP (rs2229295) in the HNF1B gene was associated with the susceptibility of T2DM. In addition, luciferase reporter assays indicate that the substitution of C>A due to SNP (rs2229295) induces the binding of hsa-miR-214-5p/hsa-miR-550a-5p to the 3′UTR of the HNF1B gene. There is a possibility that the binding of such miRNAs regulates the expression of the HNF1B gene and susceptibility of T2DM.