In vivo analysis of the canalicular trafficking of lipid transporters in lithogenic diet-fed mouse.
| Project/Area Number |
25504008
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Integrated Nutrition Science
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| Research Institution | University of Shizuoka |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 肝毛細胆管 / 細胞内トラフィキング / ＡＢＣトランスポーター / 高脂肪食 / cAMPシグナル / ABCトランスポーター / コレステロール胆石症 / protein kinase A / ABCG5 / ABCG8 / Gallstone / Liver / in vivo / Hydrodynamic injection / Trafficking / コレステロールトランスポーター / Abcg5 / Abcg8 / ABC transporter / 細胞内トラフィッキング |
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| Outline of Final Research Achievements |
ABC half transporters Abcg5 and Abcg8, which form heterodimer in the liver bile canaliculi, promote the secretion of cholesterol into bile. Hyperfunction of these transporters causes the formation of cholesterol gallstones in the bile. We previously demonstrated diet-induced hypernutrition caused a significant increase in the function and expression of these transporters in bile canaliculi, the mechanisms underlying this phenomenon remained to be clarified. In this study, we investigated how hypernutrition affects the canalicular trafficking and the function of Abcg5/Abcg8. As a result, we clarified that diet-induced cholesterol loading into liver accelerates the trafficking of Abcg5/Abcg8 from the intrahepatic pools to the bile canalicular membrane through cAMP signaling machinery.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Involvement of a Cyclic Adenosine Monophosphate-Dependent Signal in the Diet-Induced Canalicular Trafficking of Adenosine Triphosphate-Binding Cassette Transporter g5/g8.2015
Author(s)
Yamazaki, Y., Yasui, K., Hashizume, T., Suto, A., Mori, A., Murata, Y., Yamaguchi, Y., Ikari, A., Sugatani, J.
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Journal Title
Hepatology
Volume: 62
Issue: 4
Pages: 1215-1226
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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