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Chemical screening of novel factor to promote beta cell differentiation from hiPS cells

Research Project

Project/Area Number 25505003
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Regenerative medicine
Research InstitutionSaitama Medical University

Principal Investigator

Okazaki Yasushi  埼玉医科大学, 医学部, 教授 (80280733)

Co-Investigator(Renkei-kenkyūsha) Matsumoto Masahito  埼玉医科大学, 医学部, 講師 (90321819)
Research Collaborator Sugahara Yzumi (Yamashita Yzumi)  埼玉医科大学, 医学部, 研究員 (10633000)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords膵β細胞分化 / hiPSレポーター / ケミカルスクリーニング / β細胞 / single cell / double KI hiPSC / iPS細胞 / 膵β細胞 / 化合物スクリーニング
Outline of Final Research Achievements

Human induced pluripotent stem (hiPS)cells are an ideal resource for the cell based therapy. In this sense, the beta cell production by hiPS cells is a prominent therapeutic method to replenish the beta cells that are destroyed in type I diabetes. However, the efficiency and the quality of the beta cells produced from hiPS cells are not sufficient for cell therapy. The aim of this study is to improve the pancreatic beta cell differentiation from hiPS cells by screening with the chemical library. We found that the FGFR1 specific inhibitor C8 augmented the beta cell differentiation by improving the glucose responsivity and expression of the mRNA genes related to insulin releasing procedure, such as GCK, SlC30A8 and GLP1R.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (1 results)

All 2015

All Presentation (1 results)

  • [Presentation] Insulin promoter-Venus/NGN3 promoter-mCherry ダブルノックインhiPS細胞を用いたβ細胞分化誘導の解析2015

    • Author(s)
      菅原 泉, 松村瞭、佐藤達也、松谷智子、相澤絵美、岩長ゆずる、松本征仁、三谷幸之介、岡崎康司
    • Organizer
      第13回RCGMフロンティアシンポジウム
    • Place of Presentation
      埼玉医科大学 創立30周年記念講堂 (埼玉県 日高市)
    • Year and Date
      2015-10-30
    • Related Report
      2015 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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