Research for treatment of refractory arrhythmia with induced-pluripotent stem cells-derived cardiomyocytes
| Project/Area Number |
25505004
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Regenerative medicine
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| Research Institution | Keio University |
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Principal Investigator |
Jun Fujita 慶應義塾大学, 医学部, 講師 (10306706)
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| Co-Investigator(Renkei-kenkyūsha) |
FUKUDA Keiichi 慶應義塾大学, 医学部, 教授 (20199227)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 再生医療 / iPS細胞 / 再生心筋細胞 / 不整脈治療 / 不整脈 / ペースメーカー細胞 / HCN4 / 心筋細胞分化 / 細胞移植療法 / CRISPR/Cas9 / TALEN / CRISPR |
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| Outline of Final Research Achievements |
In order to advance the cell therapy for cardiac arrhythmia, massive amount of iPS Cells-derived cardiomyocytes are necessary. Therefor the massive suspension culture system with a spinner flask and efficient purification strategy of cardiomyocytes were invented. Artificial nucleases were used to insert the fluorescent proteins into the specific locus of cardiac-specific transcription factor (NKX2-5) and conduction system specific gene. These cell lines were specifically differentiated and analyzed. Purified cardiomyocytes were transplanted into pigs and the transplantation strategy was evaluated. Cardiac telemetry transmitters were also implanted into pigs and the evaluation system of arrhythmia was established.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Glutamine Oxidation Is Indispensable for Survival of Human Pluripotent Stem Cells.2016
Author(s)
Tohyama S, Fujita J, Hishiki T, Matsuura T, Hattori F, Ohno R, Kanazawa H, Seki T, Nakajima K, Kishino Y, Okada M, Hirano A, Kuroda T, Yasuda S, Sato Y, Yuasa S, Sano M, Suematsu M, Fukuda K.
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Journal Title
Cell Metab
Volume: 23
Issue: 4
Pages: 663-74
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] A massive suspension culture system with metabolic purification for human pluripotent stem cell-derived cardiomyocytes.2014
Author(s)
Hemmi N**, Tohyama S**, Nakajima K, Kanazawa H, Suzuki T, Hattori F, Seki T, Kishino Y, Hirano A, Okada M, Tabei R, Ohno R, Fujita C, Haruna T, Yuasa S, Sano M, Fujita J, Fukuda K.
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Journal Title
Stem Cells Translational Medicine
Volume: ３
Issue: 12
Pages: 1473-83
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] A Massive Floating Culture System with Metabolic Purification for Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes2014
Author(s)
Natsuko Hemmi, Shugo Tohyama, Kazuaki Nakajima, Akinori Hirano, Hideaki Kanazawa, Seki Tomohisa, Yoshikazu Kishino, Marina Okada, Ryota Tabei, Rei Ohno, Chihana Fujita, Miho Yamaguchi, Fumiyuki Hattori, Shinsuke Yuasa, Motoaki Sano, Jun Fujita, Keiichi Fukuda
Organizer
International society for stem cell research, 12th annual meeting 2014
Place of Presentation
Vancouver, Canada
Year and Date
2014-06-18 – 2014-06-21
Related Report
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[Presentation] A massive floating culture system with metabolic purification for human induced pluripotent stem cell-derived cardiomyocytes2014
Author(s)
Hemmi Natsuko, Tohyama Shugo, Nakajima Kazuaki, Hirano Akinori, Kanazawa Hideaki, Seki Tomohisa, Kishino Yoshikazu, Okada Marina, Tabei Ryota, Ohno Rei, Fujita Chihana, Yamaguchi Miho, Hattori Fumiyuki, Yuasa Shinsuke, Sano Motoaki, Fujita Jun, Fukuda Keiichi
Organizer
International society for stem cell research, 12th annual meeting
Place of Presentation
Vancouver
Related Report
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