| Project/Area Number |
25505007
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Regenerative medicine
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| Research Institution | St. Marianna University School of Medicine |
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Principal Investigator |
Suzuki Tomoko 聖マリアンナ医科大学, 医学部, 講師 (80598756)
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| Co-Investigator(Kenkyū-buntansha) |
鈴木 登 聖マリアンナ医科大学, 医学部, 教授 (40235982)
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| Co-Investigator(Renkei-kenkyūsha) |
Arimitsu Nagisa 聖マリアンナ医科大学, 医学部, 助教 (40408688)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | iPS細胞 / 再生医学 / 網膜 / 視細胞 / ロドプシン / 二次元映像 / 網膜前駆細胞 / pax6 / nrl / ケモカイン / 網膜幹細胞 / Rhodopsin陽性視細胞 / Green opsin陽性視細胞 |
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| Outline of Final Research Achievements |
The retina, the main body of light information reception, is composed of neural retina and retinal pigment epithelium. The neural retina is derived from the central nervous system, which is hard to be repaired after damage. A photoreceptor precursor cell line established by introducing pax 6 gene into mouse iPS cells differentiates into rod photoreceptor cells and cone photoreceptors with their high purity. By culturing the cell clones on a temperature sensitive gelation polymer, high purity photoreceptor cell sheet developed where they reconstructed the retinal nerve layer similar to the living body. As a result, it was possible to create a cell sheet composed of a mixture of rod photoreceptor cells and cone photoreceptor cells. By culturing the photoreceptor precursor cell sheet with various growth factors, we obtained an artificial retina sheet in which retinal ganglion cells formed synapse with photoreceptor cells.
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