Regenerative therapy for hypothalamic disorders from neoplasms
| Project/Area Number |
25505008
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Regenerative medicine
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| Research Institution | Fujita Health University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KODANI Yu 藤田保健衛生大学, 医学部, 助教 (60644622)
SUGA Hidetaka 名古屋大学, 医学部, 助教 (20569818)
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| Co-Investigator(Renkei-kenkyūsha) |
OISO Yutaka 名古屋大学, 医学部, 教授 (40203707)
OTA Akira 藤田保健衛生大学, 医学部, 教授 (10247637)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 再生医療 / 視床下部障害 / マウスES細胞 / 視床下部 / NPY / MCH / POMC / vasopressin / oxytocin / 間脳視床下部 / 視床下部機能障害 / マウス胚性幹細胞 / 中枢性肥満 |
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| Outline of Final Research Achievements |
We have executed a basic research for regenerative therapy utilizing mouse embryonal stem cell aimed at hypothalamic dysfunctions caused by brain neoplasms. First, we have developed a novel method to purify hypothalamic precursors. We have identified surface antigens on immature cells, which were removed by antibody-conjugated beads. This purification method doubled the efficacy and viability of the hypothalamic precursor cells. To improve the micro-circumstance of the grafted cells, VEGF, ROCK inhibitors and matrigel were utilized in the cell-transplantation procedure, which prolonged the grafted neurons more than a month. However, these regenerative therapies on the disease-model mice did not reach the therapeutic goals. Further research would be required to achieve the therapeutic effects.
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Report
(4 results)
Research Products
(14 results)
