| Project/Area Number |
25513002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
オミクス計測科学
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| Research Institution | Osaka University |
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Principal Investigator |
Kurono Sadamu 大阪大学, 医学(系)研究科(研究院), 准教授 (20271554)
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| Co-Investigator(Kenkyū-buntansha) |
HIEDA Miki 大阪大学, 大学院医学系研究科, 助教 (00380254)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | グライコミクス / グライコプロテオミクス / プロテオミクス / 二次元LC/MS / 絶対定量 / 選択反応モニタリング(SRM) / 安定同位体ラベル / SRM |
|---|
| Outline of Final Research Achievements |
I received 29 nipple discharges (ND) from 5 facilities of Osaka University Hospital, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka Center for Cancer and Cardiovascular Diseases Prevention, Kotsuma Clinic, and Kaizuka City Hospital. After fractionating glycoprotein from ND specimen using AAL lectin LC, I analyzed glycoproteins which exist even more abundant in breast cancer patient specimens by two-dimensional nano-LC/MS/MS, and identified a candidate protein called hornerin. After making immune rabbit antibodies using two kinds of synthetic peptides, I confirmed that one kind of antibody was preferable for reactivity with hornerin. In future, the establishment of the simple assay system of hornerin in ND using the new antibody will be required.
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