Analysis of the "epicenter of fluctuation" of proteins using mass spectrometry and the cavity omics analysis

• Project/Area Number: 25513005
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: オミクス計測科学
• Research Institution: Hiroshima University
• Principal Investigator: Izumi Shunsuke 広島大学, 理学(系)研究科(研究院), 教授 (90203116)
• Co-Investigator(Kenkyū-buntansha): Hiraoka Yasuaki 東北大学, 原子分子材料科学高等研究機構, 准教授 (10432709)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 質量分析 / タンパク質 / パーシステントホモロジー群 / HD交換速度 / トポロジカル・キャビティ / HD交換法 / オミクス計測科学 / 幾何モデル導出 / ホモロジー群 / 蛋白質内のキャビティ網羅的解析 / 位相幾何学的定量化 / 蛋白質内のキャビティの網羅的解析

Outline of Final Research Achievements

Speed of the fluctuation of the protein is measured in such as hydrogen deuterium (HD) exchange rate. In the present study, the HD exchange rate that tied the cavity of the protein (cavity), heading the relationship between the common structure of the speed and protein fluctuations, discussed the features of the HD exchange method in protein.

Research Products

• [Journal Article] Dual selective nitration in Arabidopsis: Almost exclusive nitration of PsbO and PsbP, and highly susceptible nitration of four non-PSII proteins, including peroxiredoxin II E (2015)
  • Author(s): M. Takahashi, J. Shigeto, A. Sakamoto, S. Izumi, K. Asada,d H. Morikawa
  • Journal Title: Electrophoresis Volume: 36 Issue: 20 Pages: 2569-2578
  • DOI: 10.1002/elps.201500145
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A unique mechanism of successful fertilization in a domestic bird. (2015)
  • Author(s): T. Sasanami, S. Izumi, N. Sakurai, T. Hirata, S. Mizushima, M. Matsuzaki, G. Hiyama, E. Yorinaga, T. Yoshimura, K. Ukena and K. Tsutsui
  • Journal Title: Sci. Rep. Volume: 5 Issue: 1 Pages: 7700-7700
  • DOI: 10.1038/srep07700
  • NAID: 120005527162
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] In vitro and in vivo evidence for oxalate oxidase activity of a germin-like protein from azalea (2015)
  • Author(s): Atsushi SAKAMOTO, Takashi NISHIMURA, Yoh-ichi MIYAKI, Shunsuke WATANABE, Hiroshi TAKAGI, Shunsuke IZUMI, Hiroshi SHIMADA
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 458 Issue: 3 Pages: 536-542
  • DOI: 10.1016/j.bbrc.2015.02.002
  • Peer Reviewed / Open Access
• [Journal Article] Differential regulation of epidermal growth factor receptor by hydrogen peroxide and flagellin in cultured lung alveolar epithelial cells (2015)
  • Author(s): Hiroyuki Nishi, Noriko Maeda,Shunsuke Izumi, Sayomi Higa-Nakamine, Seikichi Toku, Manabu Kakinohana, Kazuhiro Sugahara and Hideyuki Yamamoto
  • Journal Title: European Journal of Pharmacology Volume: 748 Pages: 133-142
  • DOI: 10.1016/j.ejphar.2014.12.016
  • Peer Reviewed / Open Access
• [Journal Article] Marangoni flow around a camphor disk regenerated by the interaction between camphor and sodium dodecyl sulfate molecules (2015)
  • Author(s): S. Nakata, R. Tenno, A. Deguchi, H. Yamamoto, Y. Hiraga, S. Izumi
  • Journal Title: Colloids and Surfaces A: Physicochemical and Engineering Aspects Volume: 466 Pages: 40-44
  • DOI: 10.1016/j.colsurfa.2014.10.041
  • Peer Reviewed / Open Access
• [Journal Article] Localized Bioconvection Patterns and Their Initial State Dependency in Euglena gracilis Suspensions in an Annular Container (2014)
  • Author(s): E. Shoji, H. Nishimori, A. Awazu, S. Izumi and M. Iima
  • Journal Title: J. Phys. Soc. Jpn. Volume: 83 Issue: 4 Pages: 43301-43301
  • DOI: 10.7566/jpsj.83.043001
  • NAID: 210000133019
  • Peer Reviewed / Open Access
• [Journal Article] Self-propelled Motor Driven by a Glucose Engine (2014)
  • Author(s): Yui Matsuda, Miyu Yoshii, Nobuhiko J. Suematsu, Shunsuke Izumi, Satoshi Nakata
  • Journal Title: Chemistry Letters Volume: 43 Issue: 4 Pages: 453-455
  • DOI: 10.1246/cl.131082
  • NAID: 130004868028
  • Peer Reviewed / Open Access
• [Journal Article] Alkylated Trihydroxyacetophenone as a MALDI Matrix for Hydrophobic Peptides (2013)
  • Author(s): Fukuyama,Yuko;Nakajima,Chihiro;Furuichi,Keiko;Taniguchi,Kenichi;Kawabata,Shin-ichirou;Izumi,Shunsuke;Tanaka,Koichi
  • Journal Title: Analytical Chemistry Volume: 85(20) Pages: 9444-9448
  • Peer Reviewed
• [Presentation] リパーゼの構造安定性はどのようにして決まるのか？質量分析からの一考察 (2013)
  • Author(s): 泉 俊輔
  • Organizer: 酵素工学研究会第６９回講演会
  • Place of Presentation: 名古屋大学
  • Invited
• [Patent(Industrial Property Rights)] 疎水性ペプチド用マトリックス添加剤を用いた質量分析方法 (2014)
  • Inventor(s): 泉 俊輔
  • Industrial Property Rights Holder: 泉 俊輔
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-03-18