Quantitative analysis of cellular phosphorylation signaling using electrospray ionization-mass spectrometry with an air current-regulating system
| Project/Area Number |
25513006
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
オミクス計測科学
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| Research Institution | Yokohama City University |
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Principal Investigator |
Kawakami Takao 横浜市立大学, 生命医科学研究科, 客員准教授 (40366117)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | リン酸化 / プロテオミクス / 質量分析 / プロテオーム / 分析科学 |
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| Outline of Final Research Achievements |
Quantitative and comprehensive information of posttranslational modifications of proteins is indispensable for elucidating precisely the cellular control mechanism. The optimized data analysis software improved our label-free LC-MS/MS proteomics system. We applied this system into phosphoproteomics of tumor stem cells from breast cancer. siRNA-knockdown of atypical protein kinase C lambda and zeta resulted in suppression of growth and survival of the cells, together with significant reduction of phosphorylation levels of a variety of signaling protein molecules as well as the target kinases themselves.
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Report
(5 results)
Research Products
(8 results)
