Identifications of abnormal modified proteins localized in amyloid tissues by MALDI-imaging and clarification of formation of amyloid fibril
| Project/Area Number |
25513010
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
オミクス計測科学
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| Research Institution | Osaka Medical College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
Tsuji Motomu 大阪医科大学, 医学部, 准教授 (60144466)
田窪 孝行 大阪医科大学, 医学部, 名誉教授 (60163359)
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| Co-Investigator(Renkei-kenkyūsha) |
Takubo Takayuki 大阪医科大学, 医学部, 教授 (60163359)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 質量イメージング / アミロイドーシス / 沈着分子 / コンゴレッド染色性 / 補助的病理診断 / アミロイド原性 / トランスサイレチン |
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| Outline of Final Research Achievements |
MALDI-imaging MS (IMS) with MSMS analysis is a new powerful tool for the identifications of not only disease-related proteins in formalin-fixed paraffin-embedded (FFPE) tissue section but also protein/peptides/drug/ medicine in fresh frozen tissues. This IMS used to reveal the mass profiles and spatial distribution of proteins in tissue sections and/or the digested peptides derived from the deposited protein in pathologic organs and then the MSMS analysis is identified the amino acid sequence of the detected proteins in the tissue section. Moreover, the on-tissue digestion combined with MALDI-IM-TOF-IMS approach allows a proteomics "bottom-up" strategy with clinical samples, especially perioperative isolated tissues and FFPE tissues conserved for a long time in clinical samples bank.
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Report
(4 results)
Research Products
(25 results)
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[Journal Article] Time-Course Mass Spectrometry Imaging for Depicting Drug Incorporation into Hair2015
Author(s)
Tooru Kamata, Noriaki Shima, Keiko Sasaki, Shuntaro Matsuta, Shiori Takei, Munehiro Katagi, Akihiro Miki, Kei Zaitsu, Toyofumi Nakanishi, Takako Sato, Koichi Suzuki, and Hitoshi Tsuchihashi
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Journal Title
Analytical Chemistry (ACS Publications)
Volume: -
Issue: 11
Pages: 5476-5481
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Single-hair analysis of zolpidem on the supposition of its single administration in drug-facilitated crimes2015
Author(s)
Shima N, Sasaki K, Kamata T, Matsuta S, Katagi M, Miki A, Zaitsu K, Sato T, Nakanishi T, Tsuchihashi H, Suzuki K.
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Journal Title
Forensic Toxicol.
Volume: 33
Pages: 122-130
Related Report
Peer Reviewed / Int'l Joint Research
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