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A study on a mechanism for the regulation of gene expression responding to the magnitude of gravity in plants

Research Project

Project/Area Number 25514001
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field 宇宙生命学
Research InstitutionSaitama University

Principal Investigator

KOTAKE Toshihisa  埼玉大学, 理工学研究科, 准教授 (20334146)

Co-Investigator(Kenkyū-buntansha) SOGA Kouichi  大阪市立大学, 大学院理学研究科, 准教授 (00336760)
Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords重力 / 微小管 / 細胞壁 / 重力応答 / シロイヌナズナ / アラビノガラクタン-プロテイン / 表層微小管 / MAP65 / 過重力
Outline of Final Research Achievements

Using transgenic Arabidopsis harboring genomic MAP65-1-GFP gene, the expression of MAP65-1 was analyzed in the hypocotyl. The expression level in the growing region was high whereas that in basal region was low, indicating that the expression is regulated in relation to the cell elongation. The experiments using 5’region-truncated series suggested that the region from -1.1 to -0.7 kb mainly regulates the expression in the hypocotyl. The region involved in the expression responding to the magnitude of the gravity was not identified, because stable results were not obtained.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (16 results)

All 2016 2015 2014 2013

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (12 results) Book (1 results)

  • [Journal Article] Action of an endo-b-1,3(4)-glucanase on cellobiosyl unit structure in barley b-1,3:1,4-glucan2015

    • Author(s)
      Takao Kuge, Hiroki Nagoya, Theodora Tryfona, Tsunemi Kurokawa, Yoshihisa Yoshimi, Naoshi Dohmae, Kazufumi Tsubaki, Paul Dupree, Yoichi Tsumuraya , Toshihisa Kotake
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 79 Issue: 11 Pages: 1810-1817

    • DOI

      10.1080/09168451.2015.1046365

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Action of an endo-beta-1,3(4)-glucanase on cellobiosyl unit structure in barley beta-1,3:1,4-glucan2015

    • Author(s)
      Takao Kuge, Hiroki Nagoya, Theodora Tryfona, Tsunemi Kurokawa, Yoshihisa Yoshinmi, Naoshi Dohmae, Kazufumi Tsubaki, Paul Dupree, Yoichi Tsumuraya, Toshihisa Kotake
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: -

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] β-Galactosyl Yariv reagent binds to the β-1,3-galactan of arabinogalactan-proteins2013

    • Author(s)
      Kitazawa K., Tryfona T., Yoshimi Y., Hayashi Y., Kawauchi S., Antonov L., Tanaka H., Takahashi T., Kaneko S., Dupree P., Tsumuraya Y. and Kotake T
    • Journal Title

      Plant Physiol.

      Volume: 161 Issue: 3 Pages: 1117-1126

    • DOI

      10.1104/pp.112.211722

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] In vivo degradation of sugar chian of arabinogalactan-proteins using a specific enzyme from fungi2016

    • Author(s)
      吉見圭永、吉村真美、八鍬頼誠、芝野誠二、出崎能丈、渋谷直人、円谷陽一、小竹敬久
    • Organizer
      第57回日本植物生理学会年会
    • Place of Presentation
      岩手大学、盛岡市、岩手県
    • Year and Date
      2016-03-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] 宇宙の微小重力環境下で生育したイネシュートの細胞壁変化と内生植物ホルモン量2016

    • Author(s)
      若林和幸、曽我康一、保尊隆享、小竹敬久、小嶋美紀子、榊原均、東端晃、石岡憲昭、嶋津徹、鎌田源司
    • Organizer
      第30回宇宙環境利用シンポジウム
    • Place of Presentation
      JAXA宇宙科学研究所、相模原市、神奈川県
    • Year and Date
      2016-01-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] 特異的糖鎖分解酵素を利用したアラビノガラクタン-プロテイン糖鎖の機能探索2015

    • Author(s)
      吉見圭永、吉村真美、八鍬頼誠、芝野誠二、出崎能丈、渋谷直人、円谷陽一、小竹敬久
    • Organizer
      東京糖鎖研究会
    • Place of Presentation
      慶応大学、日吉、神奈川県
    • Year and Date
      2015-10-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] Aniso Tubule宇宙実験:重力による茎の形態変化における表層微小管と微小管結合タンパク質の役割2015

    • Author(s)
      曽我康一、村上愛、小竹 敬久、加藤壮英、橋本隆、鎌田源司、山崎千秋、片平ひとみ、鈴木ひろみ、伏島康男、嶋津徹、笠原春夫、長田郁子、矢野幸子、若林和幸、保尊隆享
    • Organizer
      第29回日本宇宙生物科学会
    • Place of Presentation
      帝京大学、板橋、東京
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] MAP65とBPP1を介した重力によるシロイヌナズナ表層微小管の配向制御2015

    • Author(s)
      村上愛、曽我康一、小竹 敬久、加藤壮英、橋本隆、若林和幸、保尊隆享
    • Organizer
      第29回日本宇宙生物科学会
    • Place of Presentation
      帝京大学、板橋、東京
    • Year and Date
      2015-09-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] AGPのL-アラビノピラノース残基に作用するβ-L-アラビノピラノシダーゼ2015

    • Author(s)
      今泉知枝美、北澤仁成、戸松遥美、吉見圭永、金子哲、Dupree Paul、円谷陽一、小竹敬久
    • Organizer
      日本植物学会第79回大会
    • Place of Presentation
      朱鷺メッセ、新潟市、新潟県
    • Year and Date
      2015-09-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] アラビノガラクタンタンパク質糖鎖の人為的操作に向けた新たな試み2015

    • Author(s)
      田中 伸和、小竹 敬久
    • Organizer
      日本農芸化学会
    • Place of Presentation
      岡山大学 (岡山県岡山市)
    • Year and Date
      2015-03-29
    • Related Report
      2014 Research-status Report
  • [Presentation] Would UGE gene's overexpression directly influence an alteration of AGP polysaccharide?2015

    • Author(s)
      Tayebeh Abedi, Akio Iwasaki Toshihisa Kotake, Nobukazu Tanaka
    • Organizer
      日本農芸化学会
    • Place of Presentation
      岡山大学 (岡山県岡山市)
    • Year and Date
      2015-03-29
    • Related Report
      2014 Research-status Report
  • [Presentation] アラビノガラクタン-プロテインのコアタンパク質に作用するプロテアーゼの精製2014

    • Author(s)
      吉見圭永、菅原優美、堀千明、五十嵐圭日子、堂前直、金子哲、小竹敬久、円谷陽一
    • Organizer
      日本植物学会
    • Place of Presentation
      明治大学 (神奈川県川崎市)
    • Year and Date
      2014-09-12 – 2014-09-14
    • Related Report
      2014 Research-status Report
  • [Presentation] Purification of a protease acting on the core-protein of AGP from winter mushroom2014

    • Author(s)
      Yoshihisa Yoshimi, Yumi Sugawara, Chiaki Hori, Satoshi Kaneko, Kiyohiko Igarashi,Naoshi Dohmae, Toshihisa Kotake, Yoichi Tsumuraya
    • Organizer
      Plant Cell Wall Biology
    • Place of Presentation
      Grand Chancellor Palm Cove (オーストラリア・ケアンズ)
    • Year and Date
      2014-07-27 – 2014-07-31
    • Related Report
      2014 Research-status Report
  • [Presentation] BRITTLE CULM 4 MUTANT DECREASES IN SECONDARY CELL WALL FORMATION IN RICE2014

    • Author(s)
      Masatoshi Yamaguchi, Ami Sato, Mami Yoshimura, Tetsuya Kurata, Maki Kawai-Yamada, Shinji Kawasaki, Yoich Tsumuraya, Toshihisa Kotake
    • Organizer
      Plant Cell Wall Biology
    • Place of Presentation
      Grand Chancellor Palm Cove (オーストラリア・ケアンズ)
    • Year and Date
      2014-07-27 – 2014-07-31
    • Related Report
      2014 Research-status Report
  • [Presentation] The target structure for Yariv reagent in arabinogalactan-proteins2014

    • Author(s)
      Toshihisa Kotake, Kiminari Kitazawa, Kazuki Sato, Theodora Tryfona, Yoshihisa Yoshimi, Yoshihiro Hayashi, Susumu Kawauchi, Liudmil Antonov, Hiroshi Tanaka, Takashi Takahashi, Satoshi Kaneko, Paul Dupree, and Yoichi Tsumuraya
    • Organizer
      Plant Cell Wall Biology
    • Place of Presentation
      Grand Chancellor Palm Cove (オーストラリア・ケアンズ)
    • Year and Date
      2014-07-27 – 2014-07-31
    • Related Report
      2014 Research-status Report
  • [Book] 植物細胞壁実験法2016

    • Author(s)
      青木弾、青原勉、秋山拓也、粟野達也、安藤大将、安藤敏夫、飯山賢治、五十嵐圭日子、池正和、池田勉、石井忠、石井みどり、石水毅、磯貝明、市田淳治、伊藤瑛海、岩井宏暁、岩本訓知、上田貴志、内橋貴之、ほか
    • Total Pages
      395
    • Publisher
      弘前大学出版会
    • Related Report
      2015 Annual Research Report

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Published: 2014-07-25   Modified: 2019-07-29  

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