A study on a mechanism for the regulation of gene expression responding to the magnitude of gravity in plants

• Project/Area Number: 25514001
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: 宇宙生命学
• Research Institution: Saitama University
• Principal Investigator: KOTAKE Toshihisa 埼玉大学, 理工学研究科, 准教授 (20334146)
• Co-Investigator(Kenkyū-buntansha): SOGA Kouichi 大阪市立大学, 大学院理学研究科, 准教授 (00336760)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 重力 / 微小管 / 細胞壁 / 重力応答 / シロイヌナズナ / アラビノガラクタン-プロテイン / 表層微小管 / MAP65 / 過重力

Outline of Final Research Achievements

Using transgenic Arabidopsis harboring genomic MAP65-1-GFP gene, the expression of MAP65-1 was analyzed in the hypocotyl. The expression level in the growing region was high whereas that in basal region was low, indicating that the expression is regulated in relation to the cell elongation. The experiments using 5’region-truncated series suggested that the region from -1.1 to -0.7 kb mainly regulates the expression in the hypocotyl. The region involved in the expression responding to the magnitude of the gravity was not identified, because stable results were not obtained.

Research Products

• [Journal Article] Action of an endo-b-1,3(4)-glucanase on cellobiosyl unit structure in barley b-1,3:1,4-glucan (2015)
  • Author(s): Takao Kuge, Hiroki Nagoya, Theodora Tryfona, Tsunemi Kurokawa, Yoshihisa Yoshimi, Naoshi Dohmae, Kazufumi Tsubaki, Paul Dupree, Yoichi Tsumuraya , Toshihisa Kotake
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 79 Issue: 11 Pages: 1810-1817
  • DOI: 10.1080/09168451.2015.1046365
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Action of an endo-beta-1,3(4)-glucanase on cellobiosyl unit structure in barley beta-1,3:1,4-glucan (2015)
  • Author(s): Takao Kuge, Hiroki Nagoya, Theodora Tryfona, Tsunemi Kurokawa, Yoshihisa Yoshinmi, Naoshi Dohmae, Kazufumi Tsubaki, Paul Dupree, Yoichi Tsumuraya, Toshihisa Kotake
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: -
  • Peer Reviewed
• [Journal Article] β-Galactosyl Yariv reagent binds to the β-1,3-galactan of arabinogalactan-proteins (2013)
  • Author(s): Kitazawa K., Tryfona T., Yoshimi Y., Hayashi Y., Kawauchi S., Antonov L., Tanaka H., Takahashi T., Kaneko S., Dupree P., Tsumuraya Y. and Kotake T
  • Journal Title: Plant Physiol. Volume: 161 Issue: 3 Pages: 1117-1126
  • DOI: 10.1104/pp.112.211722
  • Peer Reviewed
• [Presentation] In vivo degradation of sugar chian of arabinogalactan-proteins using a specific enzyme from fungi (2016)
  • Author(s): 吉見圭永、吉村真美、八鍬頼誠、芝野誠二、出崎能丈、渋谷直人、円谷陽一、小竹敬久
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 岩手大学、盛岡市、岩手県
  • Year and Date: 2016-03-18
• [Presentation] 宇宙の微小重力環境下で生育したイネシュートの細胞壁変化と内生植物ホルモン量 (2016)
  • Author(s): 若林和幸、曽我康一、保尊隆享、小竹敬久、小嶋美紀子、榊原均、東端晃、石岡憲昭、嶋津徹、鎌田源司
  • Organizer: 第30回宇宙環境利用シンポジウム
  • Place of Presentation: JAXA宇宙科学研究所、相模原市、神奈川県
  • Year and Date: 2016-01-19
• [Presentation] 特異的糖鎖分解酵素を利用したアラビノガラクタン-プロテイン糖鎖の機能探索 (2015)
  • Author(s): 吉見圭永、吉村真美、八鍬頼誠、芝野誠二、出崎能丈、渋谷直人、円谷陽一、小竹敬久
  • Organizer: 東京糖鎖研究会
  • Place of Presentation: 慶応大学、日吉、神奈川県
  • Year and Date: 2015-10-24
• [Presentation] Aniso Tubule宇宙実験：重力による茎の形態変化における表層微小管と微小管結合タンパク質の役割 (2015)
  • Author(s): 曽我康一、村上愛、小竹 敬久、加藤壮英、橋本隆、鎌田源司、山崎千秋、片平ひとみ、鈴木ひろみ、伏島康男、嶋津徹、笠原春夫、長田郁子、矢野幸子、若林和幸、保尊隆享
  • Organizer: 第29回日本宇宙生物科学会
  • Place of Presentation: 帝京大学、板橋、東京
  • Year and Date: 2015-09-26
• [Presentation] MAP65とBPP1を介した重力によるシロイヌナズナ表層微小管の配向制御 (2015)
  • Author(s): 村上愛、曽我康一、小竹 敬久、加藤壮英、橋本隆、若林和幸、保尊隆享
  • Organizer: 第29回日本宇宙生物科学会
  • Place of Presentation: 帝京大学、板橋、東京
  • Year and Date: 2015-09-26
• [Presentation] AGPのL-アラビノピラノース残基に作用するβ-L-アラビノピラノシダーゼ (2015)
  • Author(s): 今泉知枝美、北澤仁成、戸松遥美、吉見圭永、金子哲、Dupree Paul、円谷陽一、小竹敬久
  • Organizer: 日本植物学会第79回大会
  • Place of Presentation: 朱鷺メッセ、新潟市、新潟県
  • Year and Date: 2015-09-07
• [Presentation] アラビノガラクタンタンパク質糖鎖の人為的操作に向けた新たな試み (2015)
  • Author(s): 田中 伸和、小竹 敬久
  • Organizer: 日本農芸化学会
  • Place of Presentation: 岡山大学 （岡山県岡山市）
  • Year and Date: 2015-03-29
• [Presentation] Would UGE gene's overexpression directly influence an alteration of AGP polysaccharide? (2015)
  • Author(s): Tayebeh Abedi, Akio Iwasaki Toshihisa Kotake, Nobukazu Tanaka
  • Organizer: 日本農芸化学会
  • Place of Presentation: 岡山大学 （岡山県岡山市）
  • Year and Date: 2015-03-29
• [Presentation] アラビノガラクタン-プロテインのコアタンパク質に作用するプロテアーゼの精製 (2014)
  • Author(s): 吉見圭永、菅原優美、堀千明、五十嵐圭日子、堂前直、金子哲、小竹敬久、円谷陽一
  • Organizer: 日本植物学会
  • Place of Presentation: 明治大学 （神奈川県川崎市）
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] Purification of a protease acting on the core-protein of AGP from winter mushroom (2014)
  • Author(s): Yoshihisa Yoshimi, Yumi Sugawara, Chiaki Hori, Satoshi Kaneko, Kiyohiko Igarashi,Naoshi Dohmae, Toshihisa Kotake, Yoichi Tsumuraya
  • Organizer: Plant Cell Wall Biology
  • Place of Presentation: Grand Chancellor Palm Cove （オーストラリア・ケアンズ）
  • Year and Date: 2014-07-27 – 2014-07-31
• [Presentation] BRITTLE CULM 4 MUTANT DECREASES IN SECONDARY CELL WALL FORMATION IN RICE (2014)
  • Author(s): Masatoshi Yamaguchi, Ami Sato, Mami Yoshimura, Tetsuya Kurata, Maki Kawai-Yamada, Shinji Kawasaki, Yoich Tsumuraya, Toshihisa Kotake
  • Organizer: Plant Cell Wall Biology
  • Place of Presentation: Grand Chancellor Palm Cove （オーストラリア・ケアンズ）
  • Year and Date: 2014-07-27 – 2014-07-31
• [Presentation] The target structure for Yariv reagent in arabinogalactan-proteins (2014)
  • Author(s): Toshihisa Kotake, Kiminari Kitazawa, Kazuki Sato, Theodora Tryfona, Yoshihisa Yoshimi, Yoshihiro Hayashi, Susumu Kawauchi, Liudmil Antonov, Hiroshi Tanaka, Takashi Takahashi, Satoshi Kaneko, Paul Dupree, and Yoichi Tsumuraya
  • Organizer: Plant Cell Wall Biology
  • Place of Presentation: Grand Chancellor Palm Cove （オーストラリア・ケアンズ）
  • Year and Date: 2014-07-27 – 2014-07-31
• [Book] 植物細胞壁実験法 (2016)
  • Author(s): 青木弾、青原勉、秋山拓也、粟野達也、安藤大将、安藤敏夫、飯山賢治、五十嵐圭日子、池正和、池田勉、石井忠、石井みどり、石水毅、磯貝明、市田淳治、伊藤瑛海、岩井宏暁、岩本訓知、上田貴志、内橋貴之、ほか
  • Total Pages: 395
  • Publisher: 弘前大学出版会