Embryonic medaka model of microglia in the developing CNS allowing in vivo analysis of their spatiotemporal recruitment in response to cosmic radiation
| Project/Area Number |
25514002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
宇宙生命学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Yasuda Takako 東京大学, 新領域創成科学研究科, 特任研究員 (40450431)
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| Co-Investigator(Renkei-kenkyūsha) |
Oda Shoji 東京大学, 新新領域創成科学研究科, 准教授 (50266714)
Funayama Tomoo 量子科学技術研究開発機構, 量子ビーム科学研究部門, 主任研究員 (40354956)
Kutsuna Natsumaro 東京大学, 新新領域創成科学研究科, 特任准教授 (70578559)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | ミクログリア / 貪食 / アポトーシス / メダカ胚 / 脳内免疫 / 放射線損傷 / 脳内免疫細胞 / 放射線 / 未熟な脳 / 胚 / 脳 / ApoE |
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| Outline of Final Research Achievements |
Here, we investigated the spatiotemporal dynamics of microglia during tissue repair in the irradiated embryonic medaka brain by whole-mount in situ hybridization using a probe for Apolipoprotein E (ApoE), a marker for activated microglia in teleosts. Three-dimensional imaging of ApoE-expressing microglia in the irradiated embryonic brain clearly showed that ApoE-expressing microglia were abundant only in the late phase of phagocytosis during tissue repair after irradiation, while few microglia expressed ApoE in the initial phase of phagocytosis. This strongly suggests that ApoE has a significant function in the late phase of phagocytosis by microglia in the medaka brain. In addition, the distribution of microglia in p53-deficient embryos at the late phase of phagocytosis was almost the same as in wild-type embryos, despite the low numbers of irradiation-induced apoptotic neurons, suggesting that constant numbers of activated microglia were recruited at the late phase of phagocytosis.
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Report
(4 results)
Research Products
(13 results)
