Detection of peptides in atmospheric organic aerosols of marine microbial origin

• Project/Area Number: 25550003
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Environmental dynamic analysis
• Research Institution: Hokkaido University
• Principal Investigator: MIYAZAKI Yuzo 北海道大学, 低温科学研究所, 助教 (60376655)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: アミノ酸 / 大気エアロゾル / 海洋微生物 / ペプチド / アミノ酸含有有機物 / 誘導体化 / タンパク質 / エアロゾル

Outline of Final Research Achievements

An analytical method was established for the determination of both free and combined amino acids including peptide in marine atmospheric aerosols. The method derivatized amino acids followed by a gas chromatography-mass spectrometry (GC-MS) analysis. The application of the adopted method in marine aerosols of the western North Pacific and the equatorial Pacific enabled the identification and quantification of 16 amino acids. Glycine was the predominant amino acids for both free and combined form in the aerosols originated from sea surface. On average, the total concentration of combined amino acids was found to be four to five times higher than that of free amino acids in the aerosol samples.

Research Products

• [Journal Article] Low-molecular-weight hydroxyacids in marine atmospheric aerosol: evidence of a marine microbial origin (2014)
  • Author(s): Y. Miyazaki, M. Sawano, and K. Kawamura
  • Journal Title: Biogeosciences Volume: 11 Issue: 16 Pages: 4407-4414
  • DOI: 10.5194/bg-11-4407-2014
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Low-molecular-weight hydroxyacids in marine atmospheric aerosol: Evidence of a marine microbial origin (2014)
  • Author(s): Y. Miyazaki, M. Sawano, K. Kawamura
  • Journal Title: Biogeosciences Discussion Volume: 11 Pages: 5743-5763
  • DOI: 10.5194/bgd-11-5743-2014
  • Peer Reviewed
• [Presentation] フィールド観測から見る陸上植生・海洋微生物起源の有機エアロゾル (2015)
  • Author(s): 宮﨑 雄三
  • Organizer: 有機エアロゾルに関するワークショップ：大気におけるその動態・性状・役割
  • Place of Presentation: 名古屋大学東京オフィス（東京）
  • Year and Date: 2015-01-23
• [Presentation] Submicrometer Water-Soluble Organic Aerosols in Marine Boundary Layer Over the Eastern Pacific (2014)
  • Author(s): Miyazaki, Y., S. Coburn, R. Volkamer, R. B. Peierce, K. Ono, and K. Kawamura
  • Organizer: NCAR/NOAA/TORERO Symposium
  • Place of Presentation: アメリカ大気研究センター（アメリカ・ボルダー）
  • Year and Date: 2014-06-24
  • Invited
• [Presentation] Ocean-derived atmospheric organic aerosols over the eastern Pacific (2013)
  • Author(s): Y. Miyazaki, R. Volkamer, R. B. Peierce, K. Ono, K. Kawamura
  • Organizer: Surface Ocean - Lower Atmosphere Study (SOLAS) Symposium
  • Place of Presentation: エポカルつくば（つくば市）
  • Invited
• [Presentation] Isotopic compositions and biogenic organic marker compounds of water-soluble organic carbon (2013)
  • Author(s): Y. Miyazaki, S. Coburn, R. Volkamer, R. B. Peierce, K. Ono, T. Takeuchi, K. Kawamura
  • Organizer: AGU Fall Meeting
  • Place of Presentation: モスコーンコンベンションセンター（アメリカ・サンフランシスコ）