| Project/Area Number |
25550019
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental dynamic analysis
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| Research Institution | National Institute for Environmental Studies |
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Principal Investigator |
IMAI AKIO 独立行政法人国立環境研究所, 地域環境研究センター, 研究センター長 (40203286)
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| Co-Investigator(Kenkyū-buntansha) |
SANO Tomoharu (独)国立環境研究所, 主任研究員 (10178808)
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| Co-Investigator(Renkei-kenkyūsha) |
KOMATSU Kazuhiro (独)国立環境研究所, 主任研究員 (20391104)
TOMIOKA Noriko (独)国立環境研究所, 主任研究員 (40168399)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 細菌生産速度測定法 / 非放射性同位体 |
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| Outline of Final Research Achievements |
We developed a nonradioactive quantitative measurement of bacterial production using stable isotope nitrogen-15-labeled deoxyadenosine (15N-dA) by liquid chromatography mass spectrometry (LC-MS). The procedures of the 15N-dA method were the incubation of seawater with 15N-dA, the filtration onto a membrane filter, the DNA extraction, the enzymatic hydrolysis of DNA to nucleosides and the quantification of 15N-dA by LC-MS. Incorporation rate of 15N-dA was significantly positively correlated to that of tritium labeled thymidine (3H-TdR) in samples of coastal seawaters. Average 15N-dA : 3H-TdR incorporation ratio was 0.55 with 2.5% and 97.5% confidence intervals of 0.51 and 0.58, respectively. The results indicated that 15N-dA could accurately predict TdR incorporations over a wide range of bacterial production.
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