Analysis of radiation-induced DNA double strand break repair during cell cycle

• Project/Area Number: 25550023
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Ibaraki University
• Principal Investigator: TAUCHI Hiroshi 茨城大学, 理学部, 教授 (70216597)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: DNA損傷修復 / 細胞周期 / DNA損傷 / DNA修復 / 相同組換え

Outline of Final Research Achievements

DNA double strand break (DSB) is one of the most serious DNA damage which is often induced by ionizing radiation. To clarify whether DSB repair occurs during just before the cell division stage or not, we developed a site- and time-specific DSB induction system. By using the system, we tried to see the DSB-localizing proteins following DNA damage induction. The experiments are still in progress, but a part of our findings has been published in a scientific journal.

Research Products

• [Journal Article] Mutations in the FHA-domain of ectopically expressed NBS1 lead to Radiosensitization and to no increase in somatic mutation rates via a partial suppression of homologous recombination (2014)
  • Author(s): Maki Ohara, Yumi Funyu, Shunsuke Ebara, Yuki Sakamoto, Ryota Seki, Kenta Iijima, Akiko Ohishi, Junya Kobayashi, Kenshi Komatsu, Akira Tachibana, and Hiroshi Tauchi
  • Journal Title: J. Radiat. Res Volume: 55 Issue: 4 Pages: 690-698
  • DOI: 10.1093/jrr/rru011
  • Peer Reviewed / Open Access
• [Journal Article] utations in the FHA-domain of ectopically expressed NBS1 lead to radiosensitization and to no increase in somatic mutation rates via a partial suppression of homologous recombination (2014)
  • Author(s): Ohara, M., Funyu, Y., Ebara, S., Sakamoto, Y., Seki, R., Iijima, K., Ohishi, A., Kobayashi, J., Komatsu, K., Tachibana, A., Tauchi, H.
  • Journal Title: Journal of Radiation Research Volume: 55
  • Peer Reviewed
• [Presentation] An experimental approach for analysis of biological effect of low dose radiation and factors affecting DSB repair fidelity (2015)
  • Author(s): Hiroshi Tauchi
  • Organizer: 30th RBC-NIRS International Symposium
  • Place of Presentation: 京都市
  • Year and Date: 2015-02-20
  • Invited
• [Presentation] 相同組換え修復の細胞周期依存性解析 (2014)
  • Author(s): 坂本裕貴、大川沙織、穀田哲也、勅使河原愛、飯島健太、高田 穣、小松賢志、田内 広
  • Organizer: 日本放射線影響学会第57回大会
  • Place of Presentation: 鹿児島
  • Year and Date: 2014-10-01
• [Presentation] DNA二重鎖切断修復欠損細胞におけるATM阻害の影響
  • Author(s): 坂本裕貴、深作直子、阿部紘子、田中 彩、戸松静香、大原麻希、高田 穣、小松賢志、立花 章、田内 広
  • Organizer: 日本放射線影響学会第56回大会
  • Place of Presentation: 青森
• [Remarks] 田内研究室ホームページ
  • URL: http://tauchilab.sci.ibaraki.ac.jp
• [Remarks] 田内研究室
  • URL: http://tauchilab.sci.ibaraki.ac.jp