| Project/Area Number |
25550024
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 放射線 / DNA損傷 / DNA修復 / 癌 / DNA二重鎖切断 |
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| Outline of Final Research Achievements |
DNA double-strand break (DSB) is considered the most critical type of DNA damage and repaired through two pathways, i.e., non-homologous end joining (NHEJ) and homologous recombination. The process and difficulty of DSB repair through NHEJ may be different depending on the structure of DNA ends, which we call “triage”. This study aimed to construct an in vitro experimental system to reproduce this phenomenon and to clarify the underlying molecular mechanism. Restriction enzyme-digested plasmids were incubated with cell extract and the product was analyzed by PCR. We established the condition of extraction, reaction and product analysis, which reflected the difference in the efficiency of repair (joining), depending on the structure of DNA ends.
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