| Project/Area Number |
25550026
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Kyoto University |
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Principal Investigator |
KOBAYASHI Junya 京都大学, 放射線生物研究センター, 准教授 (30301302)
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| Co-Investigator(Kenkyū-buntansha) |
HONDA Hiroaki 広島大学, 原爆放射線医科学研究所, 教授 (40245064)
KATO Akihiro 京都大学, 放射線生物研究センター, 研究員 (70423051)
KOMATSU Kenshi 京都大学, 放射線生物研究センター, 教授 (80124577)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | DNA修復 / 相同組換え / 非相同末端結合 / 放射線 / DNA損傷 |
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| Outline of Final Research Achievements |
In order to investigate radiosensitivity in children, we generated the system in ES cells to measure the activity of homologous recombination (HR) repair or non-homologous endjoining (NHEJ) repair. We introduce a specific site of genomic DNA to HR or NHEJ reporter construct. As a result, we got several ES cell lines, which could express NHEJ after generation of DNA double-strand break and possess differentiation potency.
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