Generation of ES cells to meseare DNA repair activity with reporter genes

• Project/Area Number: 25550026
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Kyoto University
• Principal Investigator: KOBAYASHI Junya 京都大学, 放射線生物研究センター, 准教授 (30301302)
• Co-Investigator(Kenkyū-buntansha): HONDA Hiroaki 広島大学, 原爆放射線医科学研究所, 教授 (40245064) ; KATO Akihiro 京都大学, 放射線生物研究センター, 研究員 (70423051) ; KOMATSU Kenshi 京都大学, 放射線生物研究センター, 教授 (80124577)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: DNA修復 / 相同組換え / 非相同末端結合 / 放射線 / DNA損傷

Outline of Final Research Achievements

In order to investigate radiosensitivity in children, we generated the system in ES cells to measure the activity of homologous recombination (HR) repair or non-homologous endjoining (NHEJ) repair. We introduce a specific site of genomic DNA to HR or NHEJ reporter construct. As a result, we got several ES cell lines, which could express NHEJ after generation of DNA double-strand break and possess differentiation potency.

Research Products

• [Journal Article] Mutations in the FHA-domain of ectopically expressed NBS1 lead to Radiosensitization and to no increase in somatic mutation rates via a partial suppression of homologous recombination (2014)
  • Author(s): Maki Ohara, Yumi Funyu, Shunsuke Ebara, Yuki Sakamoto, Ryota Seki, Kenta Iijima, Akiko Ohishi, Junya Kobayashi, Kenshi Komatsu, Akira Tachibana, and Hiroshi Tauchi
  • Journal Title: J. Radiat. Res Volume: 55 Issue: 4 Pages: 690-698
  • DOI: 10.1093/jrr/rru011
  • Peer Reviewed / Open Access
• [Journal Article] DNA damage signaling guards against perturbation of cyclin D1 expression triggered by low-dose long-term fractionated radiation (2014)
  • Author(s): Shimura T, Kobayashi J, Ｋomatsu K, Kunugita N
  • Journal Title: Oncogenesis Volume: 3 Issue: 12 Pages: e132-e132
  • DOI: 10.1038/oncsis.2014.48
  • Peer Reviewed / Open Access
• [Journal Article] Histone chaperone FACT regulates homologous recombination by chromatin remodeling through interaction with RNF20. (2014)
  • Author(s): Oliveira DV, Kato A, Nakamura K, Ikura T, Okada M, Kobayashi J, Yanagihara H, Saito Y, Tauchi H, Komatsu K
  • Journal Title: J Cell Sci Volume: 127 Pages: 763-772
  • DOI: 10.1242/jcs.135855
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The proteasome factor Bag101 binds to Rad22 and suppresses homologous recombination. (2013)
  • Author(s): Saito Y, Takeda J, Okada M, Kobayashi J, Kato A, Hirota K, Taoka M, Matsumoto T, Komatsu K, Isobe T.
  • Journal Title: Sci Rep. Volume: 3 Issue: 1 Pages: 2022-2022
  • DOI: 10.1038/srep02022
  • Peer Reviewed