Analysis of radiation induced mutations using next generation sequencer
| Project/Area Number |
25550030
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Hiroshima University |
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Principal Investigator |
KANAI Akinori 広島大学, 原爆放射線医科学研究所, 助教 (60549567)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAI Hidehiko 広島大学, 原爆放射線医科学研究所, 助教 (30379846)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 放射線 / 次世代シーケンサー / 1細胞 / １細胞シーケンス |
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| Outline of Final Research Achievements |
To estimate radiation induced mutation frequency with single-cell sequencing, HT1080 single cells were sorted and gamma-irradiated 0.4 Gy and 2 Gy. The cell divided several times, DNA was extracted from each single cell and we performed whole genome amplification (MALBAC method), exon capture and NGS sequence.
The number of 2 Gy specific base substitutions was 20 and indels were 3 in 7.5 Mbp. The number of 0.4 Gy specific base substitutions was 27 and indels were not detected in 14 Mbp.
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Report
(3 results)
Research Products
(7 results)
