Action-at-a-distance mutations induced by 8-oxoguanine
| Project/Area Number |
25550032
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Hiroshima University (2014)
Ehime University (2013) |
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Principal Investigator |
KAMIYA Hiroyuki 広島大学, 医歯薬保健学研究院(薬), 教授 (10204629)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | DNA損傷 / 8-oxoG / 遠隔作用変異 |
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| Outline of Final Research Achievements |
Effects of WRN- and DNA pol λ-knockdowns on mutations induced by 8-oxo-dGTP: It was suggested that WRN and DNA pol λ promote mutations induced by 8-oxo-dGTP in the nucleotide pool.
Double knockdown of WRN and DNA pol λ: The frequency of mutations induced by 8-oxoG was increased when WRN and/or DNA pol λ were knocked-down. Action-at-a-distance mutations were induced in similar levels in the double- and single-knockdown cells, suggesting that the two proteins might be located in the same pathway that suppresses the action-at-a-distance mutations.
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Report
(3 results)
Research Products
(13 results)
