Action-at-a-distance mutations induced by 8-oxoguanine

• Project/Area Number: 25550032
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Hiroshima University (2014); Ehime University (2013)
• Principal Investigator: KAMIYA Hiroyuki 広島大学, 医歯薬保健学研究院(薬), 教授 (10204629)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: DNA損傷 / 8-oxoG / 遠隔作用変異

Outline of Final Research Achievements

Effects of WRN- and DNA pol λ-knockdowns on mutations induced by 8-oxo-dGTP: It was suggested that WRN and DNA pol λ promote mutations induced by 8-oxo-dGTP in the nucleotide pool.
Double knockdown of WRN and DNA pol λ: The frequency of mutations induced by 8-oxoG was increased when WRN and/or DNA pol λ were knocked-down. Action-at-a-distance mutations were induced in similar levels in the double- and single-knockdown cells, suggesting that the two proteins might be located in the same pathway that suppresses the action-at-a-distance mutations.

Research Products

• [Journal Article] Action-at-a-distance mutagenesis induced by oxidized guanine in Werner syndrome protein-reduced human cells. (2015)
  • Author(s): H. Kamiya, D. Yamazaki, E. Nakamura, T. Makino, M. Kobayashi, I. Matsuoka and H. Harashima
  • Journal Title: Chem. Res. Toxicol. Volume: 28 Issue: 4 Pages: 621-628
  • DOI: 10.1021/tx500418m
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] DNA Polymerase λ Promotes Mutagenesis Induced by 8-Oxo-7,8-dihydroguanine (8-hydroxyguanine) Paired with Adenine (2013)
  • Author(s): H. Kamiya and M. Kurokawa
  • Journal Title: Genes and Environment Volume: 35 Issue: 4 Pages: 105-109
  • DOI: 10.3123/jemsge.2013.002
  • NAID: 10031196752
  • ISSN: 1880-7046, 1880-7062
  • Peer Reviewed
• [Presentation] 核酸を用いる発癌・制癌研究：DNA損傷による変異機構の解明と遺伝子治療用核酸の設計. (2015)
  • Author(s): 紙谷浩之
  • Organizer: 日本分析化学会中国四国支部 広島地区分析技術研究会
  • Place of Presentation: 東広島
  • Year and Date: 2015-03-18
  • Invited
• [Presentation] 遺伝子変異と損傷核酸. (2014)
  • Author(s): 紙谷浩之
  • Organizer: 日本化学会中国四国支部 愛媛地区化学講演会
  • Place of Presentation: 松山
  • Year and Date: 2014-12-24
  • Invited
• [Presentation] 8-ヒドロキシグアニン:アデニン対が誘発する変異はWerner症候群責任遺伝子産物により促進される. (2014)
  • Author(s): 紙谷浩之，中村恵里，牧野哲明，小林三和子，松岡一郎
  • Organizer: 日本環境変異原学会第43回大会
  • Place of Presentation: 東京
  • Year and Date: 2014-12-04
• [Presentation] WRN、DNA pol λノックダウン細胞における8-ヒドロキシグアニンの変異誘発. (2014)
  • Author(s): 牧野哲明，黒川正大，小林三和子，松岡一郎，紙谷浩之
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25
• [Presentation] Werner症候群責任遺伝子産物はアデニンと対合している8-ヒドロキシグアニンの変異誘発を促進する. (2014)
  • Author(s): 紙谷浩之
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-27
• [Presentation] DNA損傷が誘発する発癌機構の解明と遺伝子治療用核酸の設計. (2014)
  • Author(s): 紙谷浩之
  • Organizer: 日本分析化学会第63年会
  • Place of Presentation: 東広島
  • Year and Date: 2014-09-18
  • Invited
• [Presentation] 酸化DNA損傷による変異誘発とWRN、DNA polymerase λ. (2014)
  • Author(s): 牧野哲明，黒川正大，小林三和子，松岡一郎，紙谷浩之
  • Organizer: 第55回日本生化学会中国・四国支部例会
  • Place of Presentation: 松山
  • Year and Date: 2014-06-07
• [Presentation] DNAポリメラーゼλはアデニンと対合している8-ヒドロキシグアニンの変異誘発を促進する
  • Author(s): 紙谷浩之
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
• [Presentation] 酸化的DNA損傷による変異誘発とDNA polymerase λ
  • Author(s): 牧野哲明，黒川正大，小林三和子，松岡一郎，紙谷浩之
  • Organizer: 日本環境変異原学会第42回大会
  • Place of Presentation: 岡山
• [Presentation] 8-ヒドロキシグアニン:アデニン対が誘発する変異はDNAポリメラーゼλにより促進される
  • Author(s): 紙谷浩之，黒川正大
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] DNA polymerase λノックダウン細胞における8-ヒドロキシグアニンの変異誘発
  • Author(s): 牧野哲明，黒川正大，小林三和子，松岡一郎，紙谷浩之
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸