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Development of biological dosimetry after the irradiation.

Research Project

Project/Area Number 25550033
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Risk sciences of radiation and chemicals
Research InstitutionNagasaki University

Principal Investigator

YOSHIURA Koh-ichiro  長崎大学, 原爆後障害医療研究所, 教授 (00304931)

Research Collaborator HAYASHIDA Chisa  
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords放射線 / 変異 / 生物学的測定法 / DNA変異 / dUTP / DNAポリメラーゼ
Outline of Final Research Achievements

The aim of this research is to develop the biological dosimetry by measuring genome alterations after radiation exposure. Firstly, we developed the method for library construction from a tiny amount of DNA. DNA was amplified by using phi29 DNA polymerase with dUTP. After the amplification, 5’-end was converted to phosphate and 3’-end was to hydroxy group afer uracil DNA glycosylation and other enzymatic reactions. DNA library for sequencing was constructed from those DNA fragments for next generation sequencer. But we have not reached the step for measuring the DNA mutations after one-cell radiation exposure and for the development of biological dosimetry.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (18 results)

All 2014 2013

All Journal Article (8 results) (of which Peer Reviewed: 7 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (10 results) (of which Invited: 4 results)

  • [Journal Article] Genome-wide association study of HPV-associated cervical cancer in Japanese women.2014

    • Author(s)
      Miura K, Mishima H, Kinoshita A, Hayashida C, Abe S, Tokunaga K, Masuzaki H, Yoshiura KI.
    • Journal Title

      Journal of Medical Virology

      Volume: 86 (7) Issue: 7 Pages: 1153-1158

    • DOI

      10.1002/jmv.23943

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Heterozygous mutations in cyclic AMP phosphodiesterase-4D (PDE4D) and protein kinase A (PKA) provide new insights into the molecular pathology of acrodysostosis.2014

    • Author(s)
      Kaname T, Ki CS, Niikawa N, Baillie GS, Day JP, Yamamura KI, Ohta T, Nishimura G, Mastuura N, Kim OH, Sohn YB, Kim HW, Cho SY, Ko AR, Lee JY, Kim HW, Ryu SH, Rhee H, Yang KS, Joo K, Lee J, Kim CH, Cho KH, Kim D, Yanagi K, Naritomi K, Yoshiura KI, Kondoh T, Nii E, Tonoki H, Houslay MD, Jin DK.
    • Journal Title

      Cellular Signaling

      Volume: 26 (11) Issue: 11 Pages: 2446-2459

    • DOI

      10.1016/j.cellsig.2014.07.025

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] De novo SOX11 mutations cause Coffin-Siris syndrome2014

    • Author(s)
      Tsurusaki Y, Ohashi H, Phadke S, Koshimizu E, Kou I, Shiina M, Suzuki T, Okamoto N, Imamura S, Yamashita M, Watanabe S, Yoshiura K-i, Kodera H, Miyatake S, Nakashima N, Saitsu H, Ogata K, Ikegawa S, Miyake N, and Matsumoto N.
    • Journal Title

      Nat Commun

      Volume: 5 Issue: 1 Pages: 4011-4011

    • DOI

      10.1038/ncomms5011

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Circulating levels of maternal plasma cell-free pregnancy-associated placenta-specific microRNAs are associated with placental weight.2014

    • Author(s)
      Miura K, Morisaki S, Abe S, Higashijima A, Hasegawa Y, Miura S, Tateishi S, Mishima H, Yoshiura K, Masuzaki H.
    • Journal Title

      placenta

      Volume: 35 (10) Issue: 10 Pages: 848-851

    • DOI

      10.1016/j.placenta.2014.06.002

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ABCC11/MRP8 Expression in the Gastrointestinal Tract and a Novel Role for Pepsinogen Secretion2014

    • Author(s)
      Matsumoto H, Tsuchiya T, Yoshiura K, Hayashi T, Hidaka S, Nanashima A, Nagayasu T.
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 47 Issue: 3 Pages: 85-94

    • DOI

      10.1267/ahc.13040

    • NAID

      130004137832

    • ISSN
      0044-5991, 1347-5800
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Malfunction of Nuclease ERCC1-XPF Results in Diverse Clinical Manifestations and Causes Cockayne Syndrome, Xeroderma Pigmentosum, and Fanconi Anemia.2013

    • Author(s)
      Kashiyama, K, Nakazawa, Y, Pilz, DT, Guo, C, Sasaki, K, et al.
    • Journal Title

      The American Journal of Human Genetics

      Volume: 92 Issue: 5 Pages: 807-819

    • DOI

      10.1016/j.ajhg.2013.04.007

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Initial viral load in cases of single human papillomavirus 16 or 52 persistent infection is associated with progression of later cytopathological findings in the uterine cervix2013

    • Author(s)
      Hamaguchi D, Miura K, Abe S, Kinoshita A, Miura S, Yamasaki K, Yoshiura K, Masuzaki H
    • Journal Title

      Journal of Medical Virology

      Volume: 85 Issue: 12 Pages: 2093-2100

    • DOI

      10.1002/jmv.23709

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] 遺伝性疾患におけるエクソーム解析の有用性と近将来2013

    • Author(s)
      吉浦孝一郎
    • Journal Title

      医学のあゆみ

      Volume: 245(5) Pages: 363-368

    • Related Report
      2013 Research-status Report
  • [Presentation] 家族性肺がんにおける新規責任遺伝子の同定2014

    • Author(s)
      朝重耕一,渡辺聡,三嶋博之,木下晃,松本桂太郎,及川将弘,宮崎拓郎,土谷智史,山崎直哉,福島喜代康,永安 武,吉浦孝一郎
    • Organizer
      第59回日本人類遺伝学会
    • Place of Presentation
      タワーホール船堀(東京)
    • Year and Date
      2014-11-19 – 2014-11-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] 多発性歯牙腫合併症例を含むSATB2遺伝子変異症候群の新規変異の同定2014

    • Author(s)
      三嶋博之,菊入 崇,三古谷 忠,木下晃,吉浦孝一郎
    • Organizer
      第59回日本人類遺伝学会
    • Place of Presentation
      タワーホール船堀(東京)
    • Year and Date
      2014-11-19 – 2014-11-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] Panic障害多発家系例に対するExome解析2014

    • Author(s)
      森本芳郎,小野慎治,森 貴俊,黒滝直弘,吉浦孝一郎,小澤寛樹
    • Organizer
      第59回日本人類遺伝学会
    • Place of Presentation
      タワーホール船堀(東京)
    • Year and Date
      2014-11-19 – 2014-11-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] ddPCRを用い他McCune-Albright症候群のGNASモザイク変異検出の試み2014

    • Author(s)
      渡辺 聡,伊達木 澄人,中富明子,木下 晃,朝重耕一,木下英一,三嶋博之,森内浩幸,吉浦孝一郎
    • Organizer
      第59回日本人類遺伝学会
    • Place of Presentation
      タワーホール船堀(東京)
    • Year and Date
      2014-11-19 – 2014-11-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] 単一遺伝子病の遺伝子診断2014

    • Author(s)
      吉浦孝一郎
    • Organizer
      第9回九州遺伝子診断研究会
    • Place of Presentation
      崎大学医学部良順会館(長崎)
    • Year and Date
      2014-09-27
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 次世代シーケンサーで何が出来るのか2014

    • Author(s)
      吉浦孝一郎
    • Organizer
      第23回日本組織適合性学会大会
    • Place of Presentation
      長崎大学医学部良順会館(長崎)
    • Year and Date
      2014-09-14 – 2014-09-15
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 「予防的乳房切除の今後」 特別企画1-1 遺伝子診断が医療にもたらすもの2014

    • Author(s)
      吉浦孝一郎
    • Organizer
      第57回日本形成外科学会総会・学術集会
    • Place of Presentation
      長崎ブリックホール(長崎)
    • Year and Date
      2014-04-09 – 2014-04-11
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 「小児血液・主要研究における全エキソーム解析の可能性」 次世代シーケンサーを用いた疾患解析法~総論2014

    • Author(s)
      吉浦孝一郎
    • Organizer
      第18回小児血液セミナー
    • Place of Presentation
      ANAクラウンプラザホテル福岡(福岡市)
    • Year and Date
      2014-04-05
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Ion AmpliSeqTM Custom Panel を用いたKabuki症候群の変異解析2013

    • Author(s)
      渡辺 聡,三嶋博之,朝重耕一,木下晃,吉浦孝一郎
    • Organizer
      第58回日本人類遺伝学会
    • Place of Presentation
      江陽グランドホテル,仙台
    • Related Report
      2013 Research-status Report
  • [Presentation] 地域蓄積・収集した稀少難病の遺伝子原因究明と遺伝性疾患診断システムの構築2013

    • Author(s)
      吉浦孝一郎
    • Organizer
      公開シンポジウム-次世代遺伝子解析装置を用いた難病の原因究明,治療法開発研究プロジェクトの成果-
    • Place of Presentation
      都市センターホテル,東京
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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