Development of biological dosimetry after the irradiation.
| Project/Area Number | 25550033 |
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Research Collaborator |
HAYASHIDA Chisa
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed(Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost : ¥3,100,000、Indirect Cost : ¥930,000)
Fiscal Year 2014 : ¥2,600,000 (Direct Cost : ¥2,000,000、Indirect Cost : ¥600,000)
Fiscal Year 2013 : ¥1,430,000 (Direct Cost : ¥1,100,000、Indirect Cost : ¥330,000)
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| Keywords | 放射線 / 変異 / 生物学的測定法 / DNA変異 / dUTP / DNAポリメラーゼ |
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| Outline of Final Research Achievements |
The aim of this research is to develop the biological dosimetry by measuring genome alterations after radiation exposure. Firstly, we developed the method for library construction from a tiny amount of DNA. DNA was amplified by using phi29 DNA polymerase with dUTP. After the amplification, 5’-end was converted to phosphate and 3’-end was to hydroxy group afer uracil DNA glycosylation and other enzymatic reactions. DNA library for sequencing was constructed from those DNA fragments for next generation sequencer. But we have not reached the step for measuring the DNA mutations after one-cell radiation exposure and for the development of biological dosimetry.
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Report
(3results)
Research Products
(18results)
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[Journal Article] Heterozygous mutations in cyclic AMP phosphodiesterase-4D (PDE4D) and protein kinase A (PKA) provide new insights into the molecular pathology of acrodysostosis.2014
Author(s)
Kaname T, Ki CS, Niikawa N, Baillie GS, Day JP, Yamamura KI, Ohta T, Nishimura G, Mastuura N, Kim OH, Sohn YB, Kim HW, Cho SY, Ko AR, Lee JY, Kim HW, Ryu SH, Rhee H, Yang KS, Joo K, Lee J, Kim CH, Cho KH, Kim D, Yanagi K, Naritomi K, Yoshiura KI, Kondoh T, Nii E, Tonoki H, Houslay MD, Jin DK.
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Journal Title
Cellular Signaling
Volume : 26 (11)
Pages : 2446-2459
DOI
Related Report
Peer Reviewed
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[Journal Article] De novo SOX11 mutations cause Coffin-Siris syndrome2014
Author(s)
Tsurusaki Y, Ohashi H, Phadke S, Koshimizu E, Kou I, Shiina M, Suzuki T, Okamoto N, Imamura S, Yamashita M, Watanabe S, Yoshiura K-i, Kodera H, Miyatake S, Nakashima N, Saitsu H, Ogata K, Ikegawa S, Miyake N, and Matsumoto N.
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Journal Title
Nat Commun
Volume : 5
Pages : 4011-4011
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Malfunction of nuclease ERCC1-XPF results in diverse clinical manifestations and causes Cockayne syndrome, xeroderma pigmentosum, and Fanconi anemia.2013
Author(s)
Kashiyama, K, Nakazawa, Y, Pilz, DT, Guo, C, Sasaki, K, et al.
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Journal Title
Am J Hum Genet
Volume : 92
Pages : 1-13
DOI
Related Report
Peer Reviewed
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