| Project/Area Number |
25550037
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Japan Atomic Energy Agency |
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Principal Investigator |
YOKOYA Akinari 独立行政法人日本原子力研究開発機構, 原子力科学研究部門 先端基礎研究センター, 研究主席 (10354987)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 放射線影響 / 細胞小器官 / シナジー効果 / ミトコンドリア / X線マイクロビーム / 共生応答 / シンクロトロン放射 / タイムラプスイメージング / 放射線効果 / 細胞内小器官 / タイムラプス / 細胞ダイナミクス / ライブセルイメージング / マイクロビーム / 放射線照射効果 |
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| Outline of Final Research Achievements |
The aim of this study was to explore synergy effects of organelles in a cell exposed to ionizing radiation. We have established a technique to realize live-cell observation of morphological changes of mitochondria, which is an organelle to produce ATP. Further, we performed synchrotron X-ray microbeam irradiation to a part of a cell, and observed its membrane potential as an indicator of ATP production activity. The evidence showed that irradiating only to cytoplasm caused a delayed enhancement of mitochondrial activity, although irradiating to a cell nucleus reduced the enhancement. These results suggest that there are some synergy effects among organelles to transfer the signal of radiation hits.
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