Development of new methods that specifically detect and measure radiation-induced unrepairable DSBs
Project/Area Number |
25550038
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Risk sciences of radiation and chemicals
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Research Institution | Radiation Effects Research Foundation |
Principal Investigator |
NODA Asao 公益財団法人放射線影響研究所, 分子生物科学部, 副部長 (40294227)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | 放射線損傷 / ストレス応答 / DSB / unrepairable damages |
Outline of Final Research Achievements |
For the detection and measurement of cells bearing unrepairable DNA damages in the past-irradiated cells and tissues, we have been working on the development of new biomarkers that characterize the unrepairable damages (DNA DSBs). In this study we have found that the cells bearing the damages exhibited dysfunctional nuclear envelope structure and premature senescence. This phenotype was much more advanced in the progeria (premature aging syndrome) cells after the irradiation. By the proteome and transcriptome analyses we have identified two candidate genes (proteins) from cells carrying unrepairable DSBs. Since both proteins had ATM kinase target sequences we made antibodies against the phosphorylated forms of the proteins, and found that they showed ATM dependent phosphorylation and accumulation at the damages.
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Report
(4 results)
Research Products
(6 results)
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[Journal Article] Progerin, the protein responsible for the Hutchinson-Gilford progeria syndrome, increases the unrepaired DNA damages following exposure to ionizing radiation2015
Author(s)
Noda A, Mishima S, Hirai Y, Hamasaki K, Landes RD, Mitani H, Haga K, Kiyono T, Nakamura N, and Kodama Y
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Journal Title
Genes and Environment
Volume: 37:13
Issue: 1
Pages: 1-12
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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