| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
For the detection and measurement of cells bearing unrepairable DNA damages in the past-irradiated cells and tissues, we have been working on the development of new biomarkers that characterize the unrepairable damages (DNA DSBs). In this study we have found that the cells bearing the damages exhibited dysfunctional nuclear envelope structure and premature senescence. This phenotype was much more advanced in the progeria (premature aging syndrome) cells after the irradiation. By the proteome and transcriptome analyses we have identified two candidate genes (proteins) from cells carrying unrepairable DSBs. Since both proteins had ATM kinase target sequences we made antibodies against the phosphorylated forms of the proteins, and found that they showed ATM dependent phosphorylation and accumulation at the damages.
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