| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
To investigate the interaction potential of pharmaceuticals and personal care products (PPCPs) on fish estrogen receptor (ER) subtypes in silico, a three-dimensional model of the ER ligand-binding domain (LBD) was built and docking simulations were performed. Docking experiments revealed that medaka, zebrafish, roach, fathead minnow, carp, and stickleback ERα LBD protein strongly interacted with natural estrogens. Moreover, analgesic/anti-inflammatory, lipid regulation, β-blocker, and antidepressant drugs were docked to several fish ERα LBD. We also found differences in the key amino acid residues among the fish ER LBDs, indicating involving the differences between species and estrogenic potencies of the selected PPCPs. These results suggest that the ER signaling is disrupted by exposure to these PPCPs. Our in silico analysis may provide an insight into the potential effects of PPCPs on teleost fish.
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