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Construction of sensing systems for environmental pollutions based on biological sensing function

Research Project

Project/Area Number 25550047
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Environmental impact assessment
Research InstitutionOkazaki Research Facilities, National Institutes of Natural Sciences

Principal Investigator

AONO Shigetoshi  大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (60183729)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsセンサータンパク質 / ヘムセンサー
Outline of Final Research Achievements

In this work, the structural basis of sensor proteins was elucidated for the construction of sensitive sensing systems of environmental pollutions. Especially, the structural and functional relationships of two heme transport proteins, HupD from Listeria monocytogenes and HmuT from Corynebacterium glutamicum, that have heme sensing ability was elucidated by X-ray crystallography and spectroscopic studies to understand the molecular mechanisms of heme sensing and binding.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (11 results)

All 2014 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (10 results) (of which Invited: 1 results)

  • [Journal Article] Heme-binding properties of HupD functioning as a substrate-binding protein in a heme-uptake ABC-transporter system in Listeria monocytogenes2014

    • Author(s)
      Y. Okamoto, H. Sawai, M. Ogura, T. Uchida, K. Ishimori, T. Hayashi, S. Aono
    • Journal Title

      Bull. Chem. Soc. Jpn.

      Volume: 87 Issue: 10 Pages: 1140-1146

    • DOI

      10.1246/bcsj.20140166

    • NAID

      130004677557

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Crystal structure of HmuT reveals heme recognition2014

    • Author(s)
      Norifumi Muraki
    • Organizer
      7th Japan-Korea Seminar on Biomolecular Sciences Experiments and Simulations
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2014-11-26 – 2014-11-28
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] コリネバクテリア由来HmuTの構造とヘム認識機構2014

    • Author(s)
      村木則文
    • Organizer
      第8回バイオ関連化学シンポジウム
    • Place of Presentation
      岡山大学(岡山県岡山市)
    • Year and Date
      2014-11-11 – 2014-11-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヘム取込み系で機能するHmuTのヘム認識機構2014

    • Author(s)
      村木則文
    • Organizer
      日本結晶学会年会
    • Place of Presentation
      東京大学(東京都文京区)
    • Year and Date
      2014-11-01 – 2014-11-03
    • Related Report
      2014 Annual Research Report
  • [Presentation] Corynebacterium glutamicum由来のヘム結合タンパク質HmuTの構造と機能2014

    • Author(s)
      青野重利
    • Organizer
      第114回触媒討論会
    • Place of Presentation
      広島大学(広島県東広島市)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Structural basis for heme recognition by HmuT proteins in bacterial heme acquisition systems2014

    • Author(s)
      村木則文
    • Organizer
      第64回錯体化学討論会
    • Place of Presentation
      中央大学(東京都文京区)
    • Year and Date
      2014-09-18 – 2014-09-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] Structure and function of heme-binding proteins in heme-uptake systems of Gram-positive bacteria2014

    • Author(s)
      Shigetoshi Aono
    • Organizer
      12th European Biological Inorganic Chemistry Conference
    • Place of Presentation
      Zurich, Switzerland
    • Year and Date
      2014-08-24 – 2014-08-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] Corynebacterium glutamicum由来ヘム輸送タンパク質HmuTの結晶構造解析2014

    • Author(s)
      村木則文
    • Organizer
      第14回日本蛋白質科学会年会
    • Place of Presentation
      ワークピア横浜(神奈川県横浜市)
    • Year and Date
      2014-06-25 – 2014-06-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Corynebacterium glutamicumのヘム取込み系におけるヘム結合タンパク質HmuTの構造と機構2014

    • Author(s)
      村木則文
    • Organizer
      第41回生体分子科学討論会
    • Place of Presentation
      九州大学(福岡県福岡市)
    • Year and Date
      2014-06-06 – 2014-06-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] Listeria monocytogenes のヘム取込みに関与するHupDのヘム結合挙動の解析2013

    • Author(s)
      岡本泰典
    • Organizer
      第7回バイオ関連化学シンポジウム
    • Place of Presentation
      名古屋大学(愛知県名古屋市)
    • Related Report
      2013 Research-status Report
  • [Presentation] Biological signal transduction using heme as a signaling molecule2013

    • Author(s)
      青野重利
    • Organizer
      錯体化学会第63回討論会
    • Place of Presentation
      琉球大学(沖縄県那覇市)
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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