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Development of separation techniques for soil microbes by dielectrophoresis

Research Project

Project/Area Number 25550062
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Modeling and technologies for environmental conservation and remediation
Research InstitutionThe University of Tokyo

Principal Investigator

KURISU Futoshi  東京大学, 工学(系)研究科(研究院), 准教授 (30312979)

Co-Investigator(Kenkyū-buntansha) KASUGA Ikuro  東京大学, 大学院工学系研究科, 講師 (20431794)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords土壌微生物 / 誘電泳動
Outline of Final Research Achievements

In order to observe soil samples by microscopes, it is necessary to remove impurities. However, it is very difficult to separate the particles of the same size from bacterial cells. We applied dielectrophoresis technique to separate soil particles from bacterial cells. First we determined the optimal conditions to trap bacterial cells for two types of bacteria, and then we successfully separated bacterial cells from the soil slurry that was supplemented by the bacteria. We demonstrated the possibility to apply the technique for cell separation if we can improve recovery ratio.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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