| Project/Area Number |
25550064
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Modeling and technologies for environmental conservation and remediation
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| Research Institution | Kobe University |
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Principal Investigator |
INUI Hideyuki 神戸大学, 遺伝子実験センター, 准教授 (90314509)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | ポリ塩化ビフェニル / P450 / 代謝 / 水質汚濁・土壌汚染防止・ 浄化 |
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| Outline of Final Research Achievements |
Polychlorinated biphenyls (PCBs) show toxicity such as carcinogenicity through bioaccumulation because of its high lipophilicity and persistency. Identification of P450 monooxygenases metabolizing PCBs, creation of novel P450s with high metabolizing activities, and modification of intracellular localization of P450s were attempted to achieve remediation of PCBs in the environment.
One of PCB congeners, CB118 was metabolized by human CYP2B6 to hydroxylated metabolites due to proximity of the active center of P450s and CB118. Furthermore, P450 mutants contributed decrease of PCB toxicity, and P450s were localized in a plasma membrane.
These results showed that we established basic technologies on high metabolism of PCBs by creation of P450s with high metabolizing activities and localization of P450s.
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